The active zone is the region in the presynaptic bouton that mediates neurotransmitter release and is composed of the presynaptic membrane and a dense collection of proteins called the cytomatrix at the active zone (CAZ). The synaptic active zone contains distinct proteins such as piccolo, bassoon, SNAP25 and syntaxin. Immunostaining of the synaptic active zone utilizes presynaptic markers specific to those unique proteins.
Synaptosomal-associated protein-25 (SNAP25) is a presynaptic protein that takes part in vesicular exocytosis, neurite outgrowth, and long-term potentiation.
| Cat. No | Product Name | Reactivity | Application |
| DCABH-319 | Anti-SNAP25 monoclonal antibody, clone TQ23 | H | IHC-P, ELISA, WB |
| DPABH-16338 | Anti-Human SNAP25 polyclonal antibody | H, M, R | WB, IP, IF, ELISA |
| DCABH-8228 | Anti-SNAP25 monoclonal antibody, clone TNJ 92 | M, Ch | WB, ELISA, IHC |
Syntaxins are a family of membrane proteins primarily localized to the plasma membrane of the presynaptic active zone, of which syntaxin-1 is the primary form involved in vesicle fusion.
| Cat. No | Product Name | Reactivity | Application |
| DPAB-DC2990 | Anti-STX1A (N-terminal) polyclonal antibody | M | WB, ELISA |
| DPABH-08356 | Anti-Human STX1A polyclonal antibody | H | WB, IHC |
| DCABH-5465 | Anti-STX1A monoclonal antibody, clone FQS22184(C) | H, M, R | DCABH-5465 |
Bassoon is a large scaffolding protein of the presynaptic active zone involved in the development of presynaptic terminals and in the regulation of neurotransmitter release at both excitatory and inhibitory brain synapses.
| Cat. No | Product Name | Reactivity | Application |
| DPABH-05505 | Anti-Human BSN polyclonal antibody | H | IHC |
| DPABH-26911 | Anti-Human Bassoon polyclonal antibody | H | WB, IHC |
| CABT-B1927 | Anti-Bassoon polyclonal antibody | H, M, R | WB, IHC-P |
Piccolo is expressed during neuronal differentiation and is among the first proteins to arrive at nascent synapses, implicating it in synapse formation.
| Cat. No | Product Name | Reactivity | Application |
| DCABH-6308 | Anti-PCLO monoclonal antibody, clone QDMP12 | H | WB, FC |
| DPABH-09930 | Anti-Human PCLO polyclonal antibody | H | IHC |
By recruiting synaptic components to specific presynaptic sites, SYD-2/Liprin-α serves as a scaffolding molecule organizing the formation of functional SV release machinery. SYD-2 localizes strictly at active zones and can be used as an active zone marker.
| Cat. No | Product Name | Reactivity | Application |
| CABT-L2277 | Anti-Human PPFIA1 polyclonal antibody | H | WB |
| CABT-BL2959 | Anti-PPFIA1 (aa 574-604) polyclonal antibody | M | WB, ICC |
ELKS-1 and its homologues have been shown to localize to the AZ by immunohistochemistry and immuno-EM and interact with a variety of other AZ proteins. The physiological roles of ELKSs in neurons are complex and may differ between orthologues of different species.
| Cat. No | Product Name | Reactivity | Application |
| DPABH-09814 | Anti-Human ERC1 polyclonal antibody | H, M, R | IHC, WB, ICC-IF |
| DCABH-6839 | Anti-ERC1 monoclonal antibody, clone FQS24888 | H, M, R | WB, ICC/IF |
Species: H Human; M Mouse; R Rat; Z Zebrafish; X Xenopus laevis; B Bovine; C Cow; D Dog; P Pig; Q Quail; G Goat; Ch Chicken
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