Materials and Reagents

Description

Magic™ hybridoma growth factor is a special supplement which can successfully increase the cloning efficiency and raise the survival rate of hybridoma cell when added in the hybridoma culturing medium with no negative effect to hybridoma cells.

Key Characteristics

• Increase cloning efficiency and cell survival rate
• Culturing hybridoma cells with better antibody secretion and production
• No negative effect to hybridoma cell

Order Information

Product Data

Figure 1. Magic™ hybridoma growth factor increase hybridoma cloning efficiency. The newly PEG fused hybridoma cells were plated into a 96-well plate containing FCS media with Magic™ hybridoma growth factor (green bars), FCS media with feeder layer (blue bars) and regular FCS media (white bars). Hybridoma cells were subject to HAT selection after cell fusion. Viable colonies were counted under a microscope.

Figure 2. Magic™ hybridoma growth factor increase hybridoma growth rate. Eight clones of hybridoma cells from NS-1 or SP2/0 fusion partners were cultured in the FCS media with Magic™ hybridoma growth factor (green bars), FCS media with feeder layer (blue bars) and regular FCS media (white bars). Viable colonies in a well were counted under a microscope.

Figure 3. Magic™ hybridoma growth factor has no negative effect on titers of antibody. Hybridoma cells (anti human CD28, XPH-56) were cultured in FCS media with Magic™ hybridoma growth factor (green bars), FCS media with feeder layer (blue bars) and regular FCS media (white bars) for seven days. The titer of supernatants was examined by ELSIA assay.

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