Antigen generation services are a vital part of immunology research, therapeutic antibody development and in vitro diagnostic (IVD) assay design and development. High-quality antigens are the key to developing reliable antibodies, robust immunoassays and accurate target analyte detection. Creative Diagnostics has robust antigen generation services to support a variety of research and clinical applications.
Custom peptide synthesis, recombinant protein expression, and antigen retrieval, are all options in our antigen development platform, all fine-tuned to produce the highest purity, most stable and immunogenic products. Need antigens for immunization, assay development, or molecular characterization? Our team will work with you to determine and prepare the most appropriate antigens for your purposes.
Antigen retrieval is essential for restoring epitope accessibility in formalin-fixed paraffin-embedded (FFPE) tissues, allowing effective antibody binding in immunohistochemistry (IHC) and other assays. Our retrieval protocols include both heat-induced epitope retrieval (HIER) and enzyme-induced epitope retrieval (EIER), optimized to preserve tissue morphology while enhancing signal quality. These services are ideal for clients who are engaged in clinical pathology, biomarker validation, and translational research.
Peptide antigens are one of the best antigens in terms of specificity and flexibility, in particular for the generation of antibodies to well-defined epitopes. We offer custom peptide synthesis of high quality that include sequence design, purity optimization, and chemical modifications. In most cases peptides are conjugated to carrier proteins (KLH, BSA, OVA) to enhance immunogenicity. We also offer sophisticated conjugation strategies, designed to complement the downstream assay format. We guarantee that the synthesized peptide antigen is functional and immunogenic for reproducible antibody generation and assay development.
Recombinant protein antigens are essential reagents in both fundamental and applied biosciences. Creative Diagnostics has the capability to express your protein of interest at scale in bacterial, yeast, insect and mammalian protein expression systems. Our expression platforms can generate soluble protein, membrane protein or proteins requiring complex post-translational modification resulting in a bioactive antigen produced at high yield and correct folding. Optional custom tag insertion (His, GST, FLAG etc.) and purification is available to provide immediate downstream use in assays or antibody generation/development or therapeutic research.
Avi-tag site-specific biotinylation provides a precise and reliable approach for generating uniformly biotinylated recombinant proteins. By introducing a single biotin moiety at a defined position via BirA-mediated enzymatic labeling, this strategy preserves protein structure and biological activity while enabling controlled immobilization and sensitive detection. Our Avi-tag biotinylated proteins are well suited for ELISA, flow cytometry, SPR, BLI, and other surface-based assays requiring high reproducibility and consistent performance.
With extensive expertise in molecular biology, protein chemistry, and immunoassay development, Creative Diagnostics is your trusted partner for custom antigen generation. We are committed to delivering reproducible, application-ready antigens that meet the highest scientific standards. Our flexible service options, rapid turnaround times, and technical support ensure that your project stays on track from start to finish.
Call or email us today to talk to someone about your antigen generation needs. Detailed technical information and ordering options can be found on each service page.
