In some cases, immunizing with a peptide antigen that corresponds to a specific region of the full length protein is an ideal strategy for developing certain epitope-specific antibodies (e.g. phospho-specific). Creative Diagnostics offer a series of services, concerning sequence design, gene synthesis and peptide synthesis, to generate an appropriate peptide antigen for the antibody generation process.
For peptide antigen generation, only the amino acid sequence of the target protein is needed. Our scientist can analyze the sequence and, with the use of sophisticated algorithms (or 3D structural models), predict regions that are predicted to correspond to exposed regions of the native protein. In addition, homologous regions can be avoided, thereby helping minimize cross reactivity when assaying with the antibody.
Because the molecular weight of the peptide is not large enough to stimulate an immune response, once a peptide sequence has been chosen, it should be coupled to a carrier protein before immunizations begin. Our team can assist you to select from the following list of common carrier proteins for the Peptide Conjugation:
In order to isolate only those antibodies in the antiserum that bind with the peptide sequence, the antiserum need to be affinity purified against the peptide. This offers a significant advantage compared to immunizing with a full length protein since the resulting highly specific antibodies against the peptide sequence will approach a monoclonal antibody in specificity. For Antibody Affinity Purification, please click the link.