Neural Stem Cell Marker Antibodies

Neural stem cells (NSCs) are the stem cells of the nervous system. During development they give rise to the entire nervous system. In adults, a small number of NSCs remain and are mostly quiescent; however, ample evidence supports their important roles in plasticity, aging, disease, and regeneration of the nervous system. Understanding the extrinsic and intrinsic regulation of adult NSCs and their newborn progeny, and their response to both positive and negative stimuli will further illuminate their role in disease, injury, stress, and brain function.

The use of NSCs as a treatment strategy in CNS disease and injury has been tested for decades. Parkinsons' disease specifically has gained the most momentum for potential therapeutic benefits.

Neural Stem Cell Marker

Nestin

Nestin was originally described as a neural stem/progenitor cell marker that appeared during development of the central nervous system (CNS), and it was defined as a class VI intermediate filament protein. Nestin expression is downregulated when CNS stem/progenitor cells differentiate into neurons or glial cells. In addition to embryonic and adult CNS stem/progenitor cells, nestin is expressed in CNS tumor cells, and the degree of nestin expression correlates with the malignancy of the CNS tumor. Thus, nestin is generally recognized as a marker protein of undifferentiated CNS cells at the stage that precedes exit from the cell cycle and commitment of the mature progeny to a specific lineage.

Cat. No	Product Name	Reactivity	Application
CABT-L3001	Anti-Human Nestin monoclonal antibody, clone JID749	H	IHC
DMAB7484MH	Anti-Nestin monoclonal antibody, clone 3D14C10	H	ICC, WB
DCABH-366	Anti-NES monoclonal antibody, clone 512	M, R	ICC/IF, IHC, WB

See all Nestin antibodies

SOX2

SOX2, a HMG box transcription factor, a persistent marker for multipotential neural stem cells derived from embryonic stem cells, the embryo or the adult. SOX2 belongs to the family of SOXB1 factors and is a pivotal regulator of maintenance and induction of both PSCs and neural progenitor cells.

Cat. No	Product Name	Reactivity	Application
DMABT-H23512	Anti-SOX2 monoclonal antibody, clone Cukdf	H	FC, ELISA, IHC-P, ICC, WB
DCABH-2131	Anti-SOX2 monoclonal antibody, clone 4B3	H	WB, ELISA, IHC-P
DCABH-5662	Anti-SOX2 monoclonal antibody, clone 31H6	H, M	IHC-P, IP, WB, FC, ICC/IF

See all SOX2 antibodies

E-cadherin/CDH1

E-cadherin, a transmembrane adhesion protein, has been identified as an epithelial-to-mesenchymal transition marker and is vital for morphological structure maintenance and the functional integrity of epithelial cells.

Cat. No	Product Name	Reactivity	Application
DPAB-DC3934	Anti-CDH1 (aa 381-480) polyclonal antibody	H	WB, ELISA
DPABH-23657	Anti-CDH1 (extracellular domain) polyclonal antibody	H,M, R, P	WB
DPABH-05384	Anti-CDH1 (aa 338-649) polyclonal antibody	H	WB, ICC/IF

See all E-cadherin/CDH1 antibodies

HES1

HES1 is expressed in both neuroepithelial cells and radial glial cells, both neural stem cells. Hes1 expression, along with that of Hes5, covers the majority of the developing embryo at embryonic day 10.5. HES1 influences the maintenance of certain stem cells and progenitor cells. Specifically, HES1 influences the timing of differentiation by repressing bHLH activators, and determines binary cell fate.

Cat. No	Product Name	Reactivity	Application
CABT-L725	Anti-HES1 monoclonal antibody, clone TD17-32	H, M, R	WB, FC, ICC/IF, IHC
DPAB-DC1611	Anti-HES1 (aa 36-142) polyclonal antibody	M	WB, ELISA
CABT-RM152	Anti-HES1 monoclonal antibody, clone IT406B	H, M	IHC, ELISA

See all HES1 antibodies

HES3

Hairy and Enhancer of Split 3 (Hes3) is a recently established biomarker of neural stem cells in the fetal and adult brain of rodents and primates, including humans. Expression of Hes3 persists during quiescence, allowing the detection of non-proliferating stem cells and precursor cells. Hes3 also identifies putative cancer stem cells in aggressive brain tumors, rendering it a useful biomarker of both normal and cancerous neural stem cells.

Cat. No	Product Name	Reactivity	Application
CABT-L352R	Anti-Human HES3 polyclonal antibody	H	ELISA, IHC, ICC, IF
CABT-L351M	Anti-Human HES3 monoclonal antibody, clone 2B20	M	IP, WB
DPABH-03168	Anti-HES3 (aa 6-37) polyclonal antibody	H	IHC-P

See all HES3 antibodies

NOTCH1

NOTCH1 is one of four known genes encoding the NOTCH family of proteins, a group of receptors involved in the Notch signaling pathway. NOTCH proteins are characterized by N-terminal EGF-like repeats followed by LNR domains which form a complex with ligands to prevent signaling. Notch family members play a role in a variety of developmental processes by controlling cell fate decisions. The Notch signaling network is an evolutionarily conserved intercellular signaling pathway that regulates interactions between physically adjacent cells.

Cat. No	Product Name	Reactivity	Application
CABT-L3925	Anti-Human NOTCH1 (Cleaved-Val1754) polyclonal antibody	H, M, R	WB, IHC, ELISA
DCABH-5140	Anti-NOTCH1 monoclonal antibody, clone T364-43	M, R	WB, ICC
DCABH-9081	Anti-NOTCH1 monoclonal antibody, clone TK316	H, M	WB, ICC/IF, IHC, IP, FC

See all NOTCH1 antibodies

Clinically significant neural markers

Occludin/OCLN

Occludin was the first integral membrane protein described to be exclusively localized within tight junctions (TJs) including the blood-brain barrier (BBB) and to regulate size-selective paracellular diffusion of hydrophilic molecules. Occludin and claudin-5 form tight junction protein structures that seal the gap between the endothelial cells of the BBB and thus are responsible for maintaining the integrity of the BBB. This may make occludin vulnerable to release into the bloodstream following damage to the BBB. Recent studies have confirmed that degraded tight junction protein fragments are released into the blood and that their levels in the blood are closely related to the degree of BBB damage after cerebral ischemia. Therefore, the occludin level in peripheral blood might serve as a biomarker for cerebral edema due to BBB damage in patients with brain tumors.

Cat. No	Product Name	Reactivity	Application
DCABH-5086	Anti-OCLN monoclonal antibody, clone KK102-19	H, M, R	WB, IP, FC
DPABH-04209	Anti-Human OCLN Polyclonal antibody	H, M, R, C	WB, IP, IHC, IF, ELISA
DPABH-07559	Anti-Human OCLN Polyclonal antibody	H, M, R	WB, IP, IHC, ELISA

See all Occludin/OCLN antibodies

Species: H Human; M Mouse; R Rat; Z Zebrafish; X Xenopus laevis; B Bovine; C Cow; D Dog; P Pig; Q Quail

References

Economopoulou, M. , et al. "Expression of the neural stem cell marker Hes3 in the rodent and human ocular surface." (2013).
Frederiksen, K. , and R. Mckay . "Proliferation and differentiation of rat neuroepithelial precursor cells in vivo." Journal of Neuroscience the Official Journal of the Society for Neuroscience 8.4(1988):1144-51.
Lendahl, U. , L. B. Zimmerman , and R. Mckay . "CNS stem cells express a new class of intermediate filament protein." Cell 60.4(1990):585-595.
Shi, S. , et al. "Peripheral Blood Occludin Level as a Biomarker for Perioperative Cerebral Edema in Patients with Brain Tumors." Disease Markers 2020.3(2020):1-9.
Studer, L. . "Strategies for bringing stem cell-derived dopamine neurons to the clinic-The NYSTEM trial. " Progress in Brain Research 230(2017):191-212.
Suzuki, S. , et al. "The Neural Stem/Progenitor Cell Marker Nestin Is Expressed in Proliferative Endothelial Cells, but Not in Mature Vasculature." Journal of Histochemistry and Cytochemistry 58.8(2010):721-730.
Zhao, X. , and D. L. Moore . "Neural stem cells: developmental mechanisms and disease modeling." Cell and Tissue Research 371.1(2018):1-6.

Neuroscience Antibodies