Anti-small molecule hapten antibodies enable the detection of small molecules using robust and rapid immune detection technologies, such as ELISA, LFIA and IHC/IF. Either raised against endogenous small molecules (amino acid metabolites, lipids, saccharids, nucleotides, steroids, ...) or exogenous compounds (drug and pesticide residues, chemical pollutants,...), anti-hapten antibodies constitute attractive detection tools not only in the field of human health, for diagnostic and therapeutic applications, but also in areas such as food safety and environmental monitoring.
Combining 15 years of experience in the development of small molecule antibodies, Creative Diagnostics holds a specific expertise in the design of small molecule / protein carrier adducts. Based on our capacities in antigen design, we developed high quality small molecular monoclonal antibodies against a variety of targets.
Our advantages of custom small molecule mAbs services
|Summary of Project Proposed:|
|1||Synthesis of Immunogens||Small molecule conjugation to carrier proteins KLH for immunization. Small molecule conjugation to carrier proteins BSA for assay. Client provides small molecule to be conjugated.||1-2 weeks|
|2||Immunization||Immunization of 5 Balb/c mice with antigen (up to 4 times) and determination of antibody titer by ELISA. Animals with highest serum antibody titers are selected for fusion.||6-8 weeks|
|3||Fusion and Screening||
Fusion of spleen cells from selected mice with myeloma cell line. ELISA screening.
Selection of clones producing specific antibody.
|4||Subcloning and Generation||
Subcloning and generation of monoclonals from 3 best chosen clones.
Expansion & storage of 3 vials each of the subclones.
Production of antibodies on cell culture.
Protein G purification + ELISA validation.
Terms & Conditions:
Creative Diagnostics also offers custom services for the detection of small molecules in biological samples.