Anti-small molecule hapten antibodies enable the detection of small molecules using robust and rapid immune detection technologies, such as ELISA, LFIA and IHC/IF. Either raised against endogenous small molecules (amino acid metabolites, lipids, saccharids, nucleotides, steroids, ...) or exogenous compounds (drug and pesticide residues, chemical pollutants,...), anti-hapten antibodies constitute attractive detection tools not only in the field of human health, for diagnostic and therapeutic applications, but also in areas such as food safety and environmental monitoring.
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Combining 15 years of experience in the development of small molecule antibodies, Creative Diagnostics holds a specific expertise in the design of small molecule / protein carrier adducts. Based on our capacities in antigen design, we developed high quality small molecular monoclonal antibodies against a variety of targets.
Our advantages of custom small molecule mAbs services
Summary of Project Proposed: | |||
Phase | Summary | Description | Time |
1 | Synthesis of Immunogens | Small molecule conjugation to carrier proteins KLH for immunization. Small molecule conjugation to carrier proteins BSA for assay. Client provides small molecule to be conjugated. | 1-2 weeks |
2 | Immunization | Immunization of 5 Balb/c mice with antigen (up to 4 times) and determination of antibody titer by ELISA. Animals with highest serum antibody titers are selected for fusion. | 6-8 weeks |
3 | Fusion and Screening |
Fusion of spleen cells from selected mice with myeloma cell line. ELISA screening. Selection of clones producing specific antibody. |
3-4 weeks |
4 | Subcloning and Generation |
Subcloning and generation of monoclonals from 3 best chosen clones. Expansion & storage of 3 vials each of the subclones. |
6-7 weeks |
5 | Antibody Production |
Production of antibodies on cell culture. Protein G purification + ELISA validation. |
2-3 weeks |
Deliverables:
Terms & Conditions:
Creative Diagnostics also offers custom services for the detection of small molecules in biological samples.