Antibodies are universally considered the most important core reagents in the field of biological and medical research. In nature, however, there are few useful or specific functions for antibodies, and direct applications cannot often meet the different requirements of experiments. As a result, it is often necessary to functionally modify antibodies by labeling. Antibody labeling is the covalent or non-covalent binding of functional labels (enzymes, fluorescent dyes, biotin, metal particles, etc.) to antibody molecules, and it bestows the ability to detect, trace, purify or amplify signals.
Creative Diagnostics offer one-stop antibody labeling services, including a variety of label types and coupling strategies to improve the stability, specificity and applicability of antibodies. Labeled antibodies are widely used on a variety of experimental platforms such as ELISA, immunochromatography, flow cytometry, immunofluorescence, immunoprecipitation, molecular imaging, etc., and help researchers to powerfully push the boundaries of advanced research and diagnostic development.
We have several purification options such as Protein A/G affinity chromatography, ion-exchange, size-exclusion and antigen-specific purifications. We can purify from hybridoma supernatant, ascites or serum and provide high purity antibodies for sensitive applications like ELISA, IHC, Western blotting, and therapeutic research.
Key benefits:
For researchers seeking better tissue penetration or reduced Fc-mediated interactions, we provide enzymatic and chemical fragmentation services to generate Fab, F(ab')₂, or single-chain fragments. Each preparation undergoes purity and functionality assessments to ensure optimal performance.
Applications:
Enhance your antibody's versatility with our custom labeling services. We offer conjugation with a wide array of labels, including fluorophores (e.g., FITC), enzymes (e.g., HRP, AP), biotin, nanoparticles, and chelating agents. Site-specific conjugation options are available for controlled orientation and minimal interference with antigen binding.
Labeling formats include:
Hybridoma optimization services are available to enhance antibody expression, stability and specificity. Methods include subcloning, clone selection for highest yield, adaptation to serum free media and isotype switching. These services are a great option for laboratories seeking long-term and consistent production of a desired antibody.
Deliverables may include:
Transition seamlessly from bench to pilot or production scale with our monoclonal antibody scale-up services. We utilize both adherent and suspension cell culture systems, optimizing yield, viability, and cost-effectiveness while maintaining product quality and activity.
Capabilities:
Our team of expert scientists is intimately familiar with antibody structure and function. Building on a strong background in both immunology and molecular biology, we make sure that every modification is professional and with an eye towards increasing its value for your particular assay or therapeutic pipeline. All of our services are completely customizable, and are provided with full documentation, QC, and technical support, for complete reproducibility and consistency.
Creative Diagnostics offers full-scale, end-to-end antibody modification capabilities, from small-batch antibody labeling to large-scale custom antibody production. Our scientific and instrumentation expertise, combined with a client-focused approach to service, will help you get the results you need. Learn more about antibody modification services or contact us today to request a consultation.
