The utilization of epitope tags enables for the purification of proteins with certain tags or for the detection of proteins where specific antibodies are unavailable. Since most tag sequences are not derived from animal cells, anti-tag antibodies exhibit high reactivity with minimal non-specific binding. By eliminating the need to generate new antibodies for each protein, anti-tag antibodies also result in time, resource, and cost savings.
Creative Diagnostics offers an extensive range of high performance great value tag antibodies. Our range includes peptide and polypeptide tag antibodies, fluorescent tag antibodies, and haptens antibodies. For better economy these antibodies are available in bulk.
| Tag | Origin | Details |
| GST | Parasite (Schistosoma japonicum) | 26 kDa GST (Glutathione-S-transferase) fusion proteins can be affinity-purified using interaction with the substrate of GST, glutathione. |
| MBP | E. Coli | 43 kDa MBP (Maltose Binding Protein) fusion proteins can be purfied using an amylose column. |
| DDDK (FLAG Tag) | Artificial Sequence | 8 aa: DYKDDDDK and its variants As known as the FLAG tag. |
| His Tag | Artificial sequence | 6 aa: HHHHHH Can be used for protein purification using metal-chelating columns, such as a nickel column. This technique is suitable for low-cost, large-scale protein purification. |
| CBD | E. Coli | 29 aa: TTNPGVSAWQVNTAYTAGQLVIYNGKTYK Commonly used for protein purification. A target protein is fused to the intein and the CDB (Chitin Binding Domain). Upon induction of self-cleavage of the intein, the target protein alone is isolated. |
| CBP | Rabbit | 26 aa: KRRWKKNFIAVSAANRFKKISSSGAL CBP (Calmodulin binding protein) tightly binds to calmodulin in the presence of calcium. Because E-coli proteins do not interact with calmodulin, recombinant proteins can be obtained at high purity. |
| Strep-tag II | Artificial sequence | 8 aa: WSHPQFEK Can bind to the biotin-binding site of streptavidin. Protein purification systems using the biotin-streptavidin interaction are available. |
| T7 | Virus | 11 aa: MASMTGQQMG N-terminal peptide of the capsid protein of T7 phage. |
| HA | Virus | 9 aa: YPYDVPDYA Identified as the antibody recognition site within the envelope glycoprotein of influenza virus, hemagglutinin. |
| HSV | Virus | 9 aa: YPYDVPDYA The peptide sequence is present in the envelope protein of herpes simplex virus. |
| V5 | Virus | 14 aa: GKPIPNPLLGLDST This peptide sequence is present in the P and V proteins of Simian virus 5 (SV5) of the Rubulavirus genus in the Paramyxoviridae family. |
| VSV-G | Virus | 11 aa: YTDIEMNRLGK Vesicular stomatitis virus G glycoprotein. This peptide sequence is present in the virus envelope protein. |
| Glu-Glu | Virus | 9 aa: EEEEYMPME Polyomavirus middle T antigen |
| Myc | Human c-Myc protein | 10 aa: EQKLISEEDL c-Myc protein is a transcription factor involved in the cell cycle and apoptosis.. |
| S | Human pancreatic RNASE A | 15 aa: KETAAAKFERQHMDS The S-tag tightly binds to S-protein to form RNase S. |
| E | Osteocalcin fragment | 13 aa: GAPVPYPDPLEPR This peptide sequence is present in a bone hormone osteocalcin produced by osteoblasts. |
| Thioredoxin (Trx) | E. Coli | 12 kDa Thioredoxin is a protein involved in various oxidation-reduction reactions in the living organisms. Human cytokines and growth factors often form inclusion bodies when expressed in E. coli. Thioredoxin fusions of these proteins have higher solubility and can be purified in their active form. |
