The Molecular and Cellular Biology (MCB) laboratory toolbox would be hard-pressed to find a technique that could be considered as vital to both basic and applied research than immunoprecipitation (IP). An essential workhorse methodology that isolates and enriches a target protein and associated interaction partners from complex biological samples, IP can be performed under native or cross-linked conditions. The bait protein is captured with exquisite specificity and high affinity via antigen–antibody recognition. Immunoprecipitation is a generic, flexible enrichment method that is compatible with almost any downstream readout (Western blotting, MS, RNA-seq, chromatin mapping). Creative Diagnostics has a full range of Immunoprecipitation Services to help you perform both standard and customized molecular interaction studies.
Studying protein expression, post-translational modifications, protein–protein interactions, DNA–protein complexes or RNA-binding proteins? We offer a highly selective method to isolate defined biomolecular complexes under native or cross-linked conditions by immunoprecipitation. Validated antibodies and optimized experimental workflows make our services reproducible and sensitive for all applications. Coupling validated antibodies with optimized experimental workflows, our services are reproducible and sensitive for all applications.
Creative Diagnostics provides fully customizable IP protocols to meet the specific needs of your research. Our platform includes:
We specialize in both conventional immunoprecipitation and integrative techniques that combine IP with high-throughput molecular profiling or interaction mapping.
Our expanded menu of IP-based services are available to satisfy your experimental needs and are designed to reveal different types of biological information from cell/molecular complexes:
ChIP is used to detect protein–DNA interactions in living cells. This technique can be applied to the mapping of transcription factor binding sites, histone modifications, and epigenetic regulatory elements.
CLIP is an excellent approach for profiling RNA–protein interactions. This method can be used to generate a transcriptome-wide map of RNA binding protein (RBP) targets with nucleotide resolution.
RIP is a method used to study RNA–protein complexes. Designed to study RNA–protein complexes, RIP enables the detection of specific mRNAs or non-coding RNAs bound by RNA-binding proteins, essential in post-transcriptional regulation studies.
Ideal for studying native protein–protein interactions and complex formation under physiological conditions. Paired with MS, it offers quantitative identification of interaction partners.
The classic IP method, used to isolate and analyze individual proteins or simple complexes. Suitable for downstream Western blot, enzymatic assays, or quantification.
A dual-tag affinity strategy that provides high-purity isolation of protein complexes with minimal background. Ideal for interactome studies when paired with quantitative MS.
Our immunoprecipitation services are widely used in:
Creative Diagnostics has the expertise, instrumentation, validated reagents, and experience in protein chemistry, genomics, and transcriptomics to be your single-source provider for any immunoprecipitation based study. We offer complete, scalable, high-throughput, and publication-ready immunoprecipitation workflows that include sample preparation through to omics analysis and are optimized to your specific project needs. We can offer an isolated IP or any segment of a more complex workflow. Give us a call today to discuss your project or to request a quote for any of our customized immunoprecipitation workflows.
