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Immunocytochemistry (ICC) is a technique that allows the specific detection and localization of a particular protein or antigen in cultured cells by using antigen–antibody reactions. This is visualized through fluorescent or chromogenic markers. Immunocytochemistry is an important technique in basic research as well as clinical diagnostics. It is used to analyze the expression of proteins, their subcellular localization, and responses of cells to various treatments in situ. At Creative Diagnostics, our Immunocytochemistry Services provide high quality, reliable, customized service for your research or diagnostic application.
To further expand the power of ICC-based detection, we integrate it with complementary in situ hybridization and immunohistological techniques. These combined methodologies enable simultaneous visualization of protein expression and nucleic acid sequences within the same cellular context, significantly enhancing spatial and molecular resolution.
Explore our related specialized services:
FISH is a cytogenetic technique used by neuropathologists, cytogeneticists, and other medical professionals to identify chromosomes or parts of chromosomes in targeted tumor cells. A very sensitive technique used to detect and localize specific DNA or RNA sequences in cells through the use of fluorescently labeled probes. Suitable for chromosomal analysis, gene mapping and detection of gene amplifications or translocations.
A full service IHC offers chromogenic or fluorescence-based detection of proteins in paraffin-embedded or frozen tissue sections. This analysis is performed in the morphological context of the tissue section.
ISH detects RNA transcripts in fixed cells or tissue samples. The technique is often used to complement ICC analysis to enable gene expression analysis at the cellular level.
A two-targeted procedure that combines FISH and ICC to visualize nucleic acid sequences and protein targets in a single sample. Of particular use in cancer, stem cell and developmental studies.
Our ICC services support a wide range of applications in the life sciences, including but not limited to:
With decades of experience in immunoassay development and cellular imaging, Creative Diagnostics offers full-service ICC solutions—from antibody selection and assay optimization to imaging and data interpretation. We support a wide variety of sample types, detection systems, and multiplexing formats, ensuring that every project receives customized attention and scientifically robust results.
Key features of our ICC platform include:
From full-service support for immunocytochemistry (ICC) projects start-to-finish or the use of ICC in conjunction with FISH, IHC or ISH to provide more complete molecular profiling, Creative Diagnostics has the knowledge and experience to be your scientific partner. Our team of scientists is focused on providing you with data-driven results that are grounded in quality, reproducibility and scientific rigor.
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