Immunized host animals produce secondary antibodies when injected with antibodies from other species. This produced antibody recognizes and binds to the immunoglobulin structural area of the immunizing primary antibody, purifying, sorting and identifying the target antigen. It’s usually employed to detect and attach to specific targets on a primary antibody for signal processing and detection.
Secondary antibodies are more sensitive because they attach to multiple primary antibodies. The secondary antibody is also compatible with any other primary antibody of the same isotype and target species. And the vast majority of primary antibodies are generated in only a small number of host animals, so it is cost-effective to produce a relatively limited quantity of ready-made secondary antibodies for most approaches and detectors.
Creative Diagnostics supplies species-specific secondary antibodies with different conjugated labels. Our affinity purified secondary antibodies are available with for entire Ig classes and antibody fragments including the Fc or F(ab) region with lowest non-specific binding. The conjugated secondary antibodies are available for applications such as flow cytometry, immunohistochemistry, Western blotting, etc.
Non-Fluorescent Conjugates
They are Nanobodies/VHHs – A series of Nanobodies/VHHs that bind to primary antibodies very specifically and strongly. Nano Secondary Antibodies against rabbit and mouse IgG are now available.
High specificity and low background
One-step immunostaining protocol saves time
Recombinant antibody with high lot-to-lot consistency
Recombinant expression, does not violate animal ethics
More tissue penetration, low epitope-label displacement for higher resolution
Creative Diagnostics wants to make ADC candidate mAb search easier, and so we have created a set of secondary antibody-drug conjugates to be used for ADC-appropriate internalizing antibody screening "load-bearing" to help you in your ADC development research.
See more information on secondary antibody-drug conjugates
01
Labels
Secondary antibodies are often conjugated with labels such as enzymes (HRP and AP), fluorescent groups (FITC, TR, PE), and biotin.
02
Signal Amplification
Multiple secondary antibodies can bind to a single primary antibody, enhancing the signal and increasing detection sensitivity.
03
Flexibility
Using secondary antibodies allows for changing labels in different experiments, increasing experimental flexibility.
04
Cost-effective
Using secondary antibodies is often more economical compared to directly labeled primary antibodies.
Western blot
Immuno-histochemistry
Immuno-fluorescence
Flow cytometry 
ELISAThere is no necessary structural difference between secondary and primary antibody, both are antibodies molecules. The difference lies in their different targets.
The use of F(ab) fragments reduces nonspecific binding when the sample contains a large number of Fc receptors.
As per the kind of experiment and measurement, to select the markers - Western blot and ELISA are common HRP, immunofluorescence are common fluorescent dyes.
You can try:
Creative Diagnostics is a "One stop" antibody solutions provider serving the pharmaceutical, biotech, diagnostic and university research organizations around the world.
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![HiResNb™ Anti-Mouse IgG1 VHH antibody [Atto 488]](/upload/image/secondary-antibodies-2.jpg)
![HiResNb™ Anti-Rabbit IgG VHH antibody [Atto 488]](/upload/image/secondary-antibodies-4.jpg)
