ECL substrates solution enables highly sensitive detection of antigens through the oxidation of luminol in the presence of HRP and hydrogen peroxide. This reaction generates a stable and prolonged chemiluminescent signal, which can be detected via X-ray film or digital imaging systems.
Creative Diagnostics has continuously improved the formulations of enhancers and stabilizers, and developed two types of ECL reagents for different requirements, including UltraLume and SuperLume ECL substrates products. These products delivers a powerful, enduring signal with minimal background, allowing for extended exposure times and detection of low-abundance targets with exceptional sensitivity and precision.
| Product Name | Magic™ UltraLume ECL substrates solution | Magic™ SuperLume ECL substrates solution |
| Applicable Samples | Extremely low abundance target proteins | Low abundance target proteins |
| Advantages | Highest sensitivity | Long signal duration |
| Sensitivity | Low-femtogram | Picogram |
| Signal Duration | 6 h | ~12 h |
| Dilution of primary Ab | 1:5,000-100,000 | 1:1,000-50,000 |
| Dilution of Secondary Ab | 1:100,000-250,000 | 1:50,000-250,000 |
| Membrane | PVDF / Nitrocellulose | PVDF / Nitrocellulose |
| Storage Stability | 4°C for 1 year, RT for 6 months | 4°C for 2 year, RT for 1 year |
