Microglia Markers Antibodies

Microglia are cells of mesodermal/mesenchymal origin that migrate into the CNS to become resident macrophages within the unique brain microenvironment. Microglia are highly dynamic cells that interact with neurons and nonneuronal cells.

Microglia express a wide array of receptors and thus respond to pleiotropic stimuli ranging from neurotransmitters to cytokines and plasma proteins. They play a crucial role in the healthy brain as regulators of synaptic functions and phagocytosis of newborn neurons, with important implications in synaptic plasticity and adult neurogenesis. In disease, they play a crucial role in neurodegenerative and neuroinflammatory conditions. Their interactions with T cells are a major component of the development of brain autoimmunity, while their pathogenic interactions with neurons via induction of ROS and iNOS play a crucial role in neurodegeneration.

Microglia Markers

AIF-1/Iba1

Ionized calcium-binding adapter molecule 1 (Iba1) is a 147-amino-acid calcium-binding protein widely in use as a marker for microglia. It has actin-crosslinking activity and is involved in aspects of motility-associated rearrangement of the actin cytoskeleton.

Cat. No	Product Name	Reactivity	Application
DPABH-03784	Anti-AIF1 (aa 1-147) polyclonal antibody	H, M	WB, IHC, FC
CPBT-65002GA	Anti-AIF1 (C-terminal) polyclonal antibody	H, M, R	WB, IHC-P, ELISA
CABT-12622MH	Anti-AIF1 monoclonal antibody, clone 3B3-C7	H	WB, ELISA

See all AIF-1/Iba1 antibodies

CD11b/ITGAM

CD11b is an integrin family member which pairs with CD18 to form the CR3 heterodimer. CD11b is expressed on the surface of many leukocytes including monocytes, neutrophils, natural killer cells, granulocytes and macrophages, as well as on 8% of spleen cells and 44% of bone marrow cells. It is an excellent marker of macrophages and microglia.

Cat. No	Product Name	Reactivity	Application
DCABH-9069	Anti-ITGAM monoclonal antibody, clone FQ2456Z	H	WB, IP, ICC, FC, IHC
DPAB-DC1743	Anti-ITGAM (aa 111-220) polyclonal antibody	H	WB, ELISA
DPABH-22990	Anti-ITGAM (internal region) polyclonal antibody	H, M, R	IHC-P, ICC/IF

See all CD11b/ITGAM antibodies

CX3CR1

CX3CR1 is a fractalkine receptor which is expressed specifically by microglia in the brain parenchyma.

Cat. No	Product Name	Reactivity	Application
DCABH-7911	Anti-CX3CR1 monoclonal antibody, clone 9F21.E0	H	WB, IHC-P
CPBT-65370RH	Anti-CX3CR1 (aa 175-189) polyclonal antibody	H	WB, FC
CABT-L1260	Anti-Human CX3CR1 monoclonal antibody, clone 2I25M8	H, M, R	WB, ICC/IF

See all CX3CR1 antibodies

EMR1, F4/80

EGF-like module-containing mucin-like hormone receptor-like 1 also known as F4/80 is a protein encoded by the ADGRE1 gene. It has been widely-used as a monocyte-macrophage marker in mice.

Cat. No	Product Name	Reactivity	Application
CABT-L4474	Anti-Mouse F4/80 Monoclonal antibody, clone CI:A3-1	M	FuncS, IHC-P, IHC-F, FC
DCABH-6003	Anti-EMR1 monoclonal antibody, clone G5/91	M	WB, ICC/IF
DCABH-15300	Anti-Mouse F4/80 monoclonal antibody, clone TQ226	H, M	IHC-P

See all EMR1, F4/80 antibodies

Fcγ RI/CD64

CD64 is a 72 kD single chain type I glycoprotein also known as FcγRI and FcRI. It is a granulo-monocytic lineage marker on CD34+ hematopoietic progenitor cells.

Cat. No	Product Name	Reactivity	Application
CABT-L2076	Anti-Human FcgRI polyclonal antibody	H	WB
DCABH-6301	Anti-Human CD64 (FCGR1) Monoclonal Antibody, clone 10.1	H	BL, FA, FC, IHC

See all Fcγ RI/CD64 antibodies

FCRL4/FcRH4

FcRL4 is a transmembrane protein expressed on a subset of memory B cells. It has been indicated that FCRLs may be a unique marker for microglia in the brain.

Cat. No	Product Name	Reactivity	Application
CABT-BL140	Anti-FCRL4 polyclonal antibody	H, M, R	WB
CABT-BL1505	Anti-FCRL4 (aa 35-84) polyclonal antibody	H	WB

See all FCRL4/FcRH4 antibodies

M-CSF 1R/CD115

CD115 (macrophage colony-stimulating factor receptor, or CSF-1 receptor) is the sole cell-surface receptor identified for CSF-1, the predominant growth factor regulating the survival, growth and differentiation of myeloid lineage cells comprising monocytes, macrophages, DCs and osteoclasts. CD115 has been proposed as a marker for the standardized identification and analysis of mouse monocytes.

Cat. No	Product Name	Reactivity	Application
CABT-L3452	Anti-Human CSF1R (Phospho-Tyr561) polyclonal antibody	H, M, R	WB, ELISA, IHC
CABT-BL7399	Anti-CSF1R monoclonal antibody, clone BGT09	M	FC, IHC, IHC-F, FA, WB
CABT-L4320	Anti-Mouse CSF1R (CD115) monoclonal antibody, clone AFS98	M	Neut, FC, WB

See all M-CSF 1R/CD115 antibodies

P2RY12

Purinergic Receptor P2Y12 (P2RY12), a P2 purinergic receptor, is introduced as a specific marker for microglial cells under normal and pathologic conditions. P2RY12 belongs to the family of P2 purinergic receptors, consisting of seven transmembrane G protein coupled receptors (GPCRs) that contribute to ATP-and ADP-mediated cell migration in vitro. P2RY12 is expressed in activated platelets and notoriously, in microglial cells.

Cat. No	Product Name	Reactivity	Application
DPABH-14273	Anti-P2RY12 (aa 11-24) polyclonal antibody	H, M, R	WB, IHC-P
CABT-L679M	Anti-Human P2RY12 Monoclonal Antibody, clone 1C2A9	H	WB, IHC, ICC, ELISA
DCABH-12761	Anti-P2RY12 monoclonal antibody	H	WB, ELISA

See all P2RY12 antibodies

Siglec-H

Sialic acid-binding immunoglobulin-like lectin H (Siglec-H) is expressed by parenchymal microglia from developmental stages to adulthood, and the expression was maintained in activated microglia under injury or inflammatory condition. Siglec-H expression was absent from CNS-associated macrophages and CNS-infiltrating monocytes, except for a minor subset of cells. It is a microglia-specific marker that discriminates microglia from CNS-associated macrophages and CNS-infiltrating monocytes.

Cat. No	Product Name	Reactivity	Application
CABT-BL3294	Anti-SIGLECH monoclonal antibody, clone 550d	M	FC, IHC-Fr, Inhib
CABT-L4471	Anti-Mouse Siglec-H Monoclonal antibody, clone 440c	M	FC

See all Siglec-H antibodies

TMEM119

TMEM119 is expressed on immortalized human microglia, in which the expression levels are not elevated by exposure to lipopolysaccharide, IFNγ, IL-4, IL-13 or TGFβ1. It is a microglia-specific marker in both mouse and human CNS.

Cat. No	Product Name	Reactivity	Application
DPABH-14818	Anti-TMEM119 (aa 194-282) polyclonal antibody	H	IHC-P
DPABH-14822	Anti-TMEM119 (aa 117-167) polyclonal antibody	H	IHC-P

See all TMEM119 antibodies

TREM2

TREM2 is a receptor glycoprotein that belongs to the immunoglobulin superfamily. In the brain, TREM2 is expressed exclusively by myeloid cells, including microglia and macrophages. Its expression in the brain is upregulated in response to the tissue damage that accumulates in aging and in neurodegenerative diseases. It is a marker of microglial activation.

Cat. No	Product Name	Reactivity	Application
CABT-B8535	Anti-TREM2 (C-terminal) polyclonal antibody	M	ELISA, IF, WB
CABT-L213R	Anti-Human TREM2 polyclonal antibody	H, M	WB, ELISA
CABT-47854RM	Anti-TREM2 monoclonal antibody, clone 78.18	M	FC, FA

See all TREM2 antibodies

Species: H Human; M Mouse; R Rat; Z Zebrafish; X Xenopus laevis; B Bovine; C Cow; D Dog; P Pig; Q Quail; G Goat

References

"A unique anti-CD115 monoclonal antibody which inhibits osteolysis and skews human monocyte differentiation from M2-polarized macrophages toward dendritic cells." mAbs (2013).
Changbin, et al. "Expression site of P2RY12 in residential microglial cells in astrocytomas correlates with M1 and M2 marker expression and tumor grade." Acta Neuropathologica Communications 5.1(2017).
Magnus, et al. "CSF concentrations of soluble TREM2 as a marker of microglial activation in HIV-1 infection. " Neurology Neuroimmunology & Neuroinflammation (2019).
Olweus, J. , F. Lundjohansen , and L. W. Terstappen . "CD64/Fc gamma RI is a granulo-monocytic lineage marker on CD34+ hematopoietic progenitor cells." Blood 85.9(1995):2402.
Rubenstein, J. . Patterning and Cell Type Specification in the Developing CNS and PNS. 2013.
Satoh, J. I. , et al. "TMEM119 marks a subset of microglia in the human brain." Neuropathology 36.1(2016):39-49.

Neuroscience Antibodies