Creative Diagnostics staff scientists have extensive antibody purification experience. We have developed robust proprietary purification procedures for antibodies from serum, ascites fluid, yolks and culture supernatants for use in early discovery research, preclinical trials and in vitro medical devices. All purified antibodies can be analyzed by electrophoresis, ELISA, western blot and HPLC to determine purity and integrity. Corresponding test data will be provided to the customer.
Antigen/Antibody affinity purification
Antigen/Antibody affinity can be used to obtain extremely pure specific antibodies from complex samples. Due to the highly specific purification process, the resulting antibody preparation tends to have lower background levels and lower non-specific binding rates. Our scientists have generated various affinity columns, like target-specific peptides, recombinant proteins and antibody based column, and employed several key procedures to provide the best possible affinity purified antibodies.
Additional service: Agarose Bead Conjugation
Our agarose and cross-linked agarose bead conjugation services offer conjugations to a variety of molecules, including proteins, peptides, antibodies and numerous small molecules with reactive groups for using in purification and immuno-precipitation processes
Protein A, G, or A/G Affinity
Protein A and Protein G are the most common choices for antibody purification due to their ability to bind the constant (Fc) region of IgG from various species. Since Protein A and Protein G have differing binding efficiencies for IgG from different species, it is important to check this before deciding which method to use. Protein A/G, a recombinant fusion protein that combines IgG binding domains of both Protein A and Protein G, has the additive properties of Protein A and G.
Protein L Affinity
Protein L binds antibodies through kappa light chain interactions. Protein L binds a wider range of antibody classes than Protein A or G, including ScFv and Fab fragments. Protein L is extremely useful for purification of antibodies from culture supernatant because it does not bind bovine immunoglobilins, which are often present in the media as a serum supplement. Also, Protein L does not interfere with the antigen-binding site of the antibody, making it useful for immunoprecipitation assays, even using IgM.
IgY Purification from Egg Yolk
Chickens produce unique antibodies called IgY. Our team has developed multi-step purification, concerning PEG precipitation, ion exchange and Antigen/ Protein L affinity chromatography, to purify and enrich specific IgY antibodies from chicken egg yolk. Typically, we need to test the starting material by ELSIA assay and run SDS-PAGE to analysis the final purified antibodies.
Low Endotoxin Purification
Endotoxins, toxic substance bound to the bacterial cell wall and released when the bacterium ruptures or disintegrates. Removal of endotoxin is one of the most difficult downstream processes during antibody purification, because endotoxin is extremely heat and pH stable. Our team has developed a low endotoxin purification process that serves to minimize endotoxin loads. Besides, our rapid test methods allow us to produce results right at the point of antibody purification.
We also offer following services, which could be utilized dependently or independently, to support our customs’ antibody purification program.
Size Exclusion/ Gel Filtration Chromatography
Welcome to discuss your antibody purification options with our expert teams. We will help you find or develop the optimal antibody purification procedure to reach the best balance between yield, purity and the cost.