Magic Fast™ ALP Conjugation Kit - Creative Diagnostics

Creative Diagnostics has designed the Magic Fast™ ALP conjugation kit to enable the direct labeling of any antibodies, antibody fragments, proteins, peptides or other biomolecules with an available amine group to accelerate life science fundamental research and the development of the diagnostic kit. Our technology can be used to label both small (e.g. 50 µg) and large quantities of biomolecules with ease. The researcher simply pipettes their materials into the vial and incubates for 3 hours.

Performance

Material selection: Any proteins, antibodies, antibody fragments, peptides or other biomolecules with available amine groups.
Reactivity: free -NH2.
Linkage stability: Activation of proprietary reagents within the offered solution results in directional covalent bonding of ALP to the biomolecules.
East-to-go: By circumventing desalting or dialysis steps that commonly interrupt traditional antibody conjugation procedures, the researchers get the conjugated biomolecules after simply mixing the materials together.
Yield: Circumvention of the purification steps makes our Magic Fast™ ALP conjugation kit can reach 100% recovery and allows materials to start at small quantities (e.g. 50 µg).
Rate: Instant reaction; the whole process less than 3 hours.
Consistence: Good reproducibility from batch to batch.

Kit Components

Solution A: Catalyzer solution (1 vial)
Solution B: Activated ALP solution (10 mg/ml, 1 vial)
Solution C: Stabilizer

Cat.	Amount of Antibody	Solution A	Solution B	Solution C
CD4005-100µg	100 µg	20 ul	12 µl	1 ml
CD4005-500µg	500 µg		60 µl	5 ml
CD4005-1mg	1 mg		120 µl	10 ml
CD4005-5mg	5 mg	40 ul	600 µl	50 ml
Note: The kit above is designed for antibodies, but works well for any amine-containing biomolecule. Please follow same technical tips if required.

Recommended Application Notes

Suggested optimal concentration for antibody conjugation is 4-6 mg/ml.
The recommended conjugation ratio of antibody to ALP is 1:0.8.
Solution A (µl) / Unconjugated material (ug): 0.75 µl / 100 µg.
Solution B (µg) / Unconjugated material (ug): 80 µg / 100 µg.
Solution C (ml) / Conjugated material (µg): 1 ml / 100 µg.

Each laboratory should determine an optimum amount for use in its particular application.

Buffer Considerations

Buffer Components & Conditions
Amine free buffer (e.g HEPES, MES, MOPS and phosphate)	Yes
Borate buffer	Yes
Chelating agents (e.g. EDTA)	Yes
Glycerol	No
Glycine	No
Material supplied in ascites fluid, serum, hybridoma or tissue culture media	No
Merthiolate	No
Non-buffering salts (e.g. sodium chloride)	Yes
Other biomolecules	Primary amines required
Peptides	Yes
pH	6.0-8.5
Proclin	No
Proteins	Yes
Purified antibody	Yes
Sodium Azide	No
Sugars	Yes
Thiomersal / Thimerosal	No
Tris	No

Storage

Solution A: Stable at 4℃ for 1 week. Stable at -20℃ for 18 months.
Solution B: Stable at -20℃ for 18 months.
Conjugated Materials: Stable at 2-8 for 12 months if Solution C is added.

Assay Procedure

Equilibrate all materials and prepared reagents to room temperature prior to use. (Solution A should be thoroughly mixed after it has completely melted.)
Add 80 µg of Solution B (Activated ALP solution) to each 100 µg of antibody to be labeled and mix gently.
Add 0.75 µl of Solution A (Catalyzer solution) to each 100 µg of antibody to be labeled and mix gently.
Replace cap on the vial and leave standing for 3 hours in the dark at room temperature (20-25℃). Longer incubation times, such as overnight at 4℃ is also recommended and have no negative effect on the conjugation.
After incubating, the conjugated reagent is ready to use. This conjugation do not require purification.

Figure 1. Assay Procedure of Magic Fast™ ALP Conjugation Kit

Note: The kit above is designed for antibodies, but works well for any amine containing biomolecule. Please follow same technical tips if required.

Cat No.	Product name
CD4005-100µg	Magic Fast™ ALP Conjugation Kit (100 ug)	Inquiry
CD4005-500µg	Magic Fast™ ALP Conjugation Kit (500 ug)	Inquiry
CD4005-1mg	Magic Fast™ ALP Conjugation Kit (1mg)	Inquiry
CD4005-5mg	Magic Fast™ ALP Conjugation Kit (5mg)	Inquiry
Note: The kit above is designed for antibodies, but works well for any amine containing biomolecule. Please follow same technical tips if required.