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Our Innovative Protein Expression Platform integrates advanced expression technologies and host systems to deliver high-quality recombinant proteins with authentic conformation and superior bioactivity. We specialize in complex viral glycoproteins, multimeric antigens, and membrane-associated proteins, meeting the demands of vaccines, diagnostics, and structural biology.
To overcome the inherent challenges of membrane and multimeric protein production, we employ a series of optimized strategies at multiple levels to significantly improve protein stability and yield.
Sequence Optimization: We enhance translation efficiency through codon optimization while performing rational truncation of non-functional or aggregation-prone regions to retain essential domains. Fusion tags are introduced when needed to improve solubility, stability and downstream purification.
Host Selection: Mammalian expression systems such as HEK293 and CHO are prioritized to ensure authentic folding, disulfide-bond formation and post-translational modifications. In parallel, insect, yeast or bacterial systems are selected strategically to address diverse production requirements.
Vector Engineering: High-efficiency cis-regulatory elements are incorporated into expression vectors to boost transcription and mRNA stability. For difficult-to-express proteins, multi-copy or custom vectors can increase yields by 100–400%. Co-expression inhibitors or specific ligands are applied to reduce cell toxicity and stabilize protein conformation.
Process Optimization: Specialized additives are supplemented during cell culture to enhance tolerance to selection pressure, achieving up to threefold higher yields. Gentle orbital-shaking bioreactors further minimize shear stress, prolong cell viability and increase total output while maintaining protein integrity.
Each recombinant protein is subjected to a comprehensive panel of analytical assays to verify identity, purity, structural integrity, and biological activity:
SDS-PAGE and Western Blotting for size and identity verification
ELISA and Binding Assays for antigenicity and epitope preservation
SEC-MALS and Mass Spectrometry for oligomerization state, molecular weight, and post-translational modification profiling
Glycosylation and Functional Activity Analyses to ensure authentic structural features.
Our recombinant proteins are widely used in:
Vaccine Research and Development - High-fidelity antigens for immunization studies
Diagnostic Assay Development - Matched antigen pairs for ELISA, rapid tests, and multiplex platforms
Structural Biology - Cryo-EM or X-ray crystallography of complex membrane proteins
Antibody Discovery and Screening - Authentic targets for monoclonal and nanobody screening
Our platform offers high expression yields with scalable production capability, authentic conformation and antigenicity through optimized design and host selection, comprehensive quality control ensuring reproducibility and regulatory compliance, rapid turnaround from gene to purified protein, and custom development with full technical support including OEM/ODM services.
By combining advanced molecular design with robust expression technologies, our platform delivers reliable, high-quality proteins—even for the most challenging membrane-associated targets—accelerating your research, diagnostic and vaccine development programs.
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