Matched antibody pairs consist of two different antibodies generated against the same target, but with different binding regions or epitopes. Differential epitope recognition allows for simultaneous binding of both antibodies to the same target. They can be used in assays such as sandwich ELISA to detect the antigen by immobilizing one antibody on a substrate to capture the antigen while the second antibody is labeled to detect the captured antigen. In some cases, an antibody may self pair.
With multiple antibody pair screening platforms, Creative Diagnostics can determine whether antibodies we have developed for you and/or antibodies you have supplied bind to different epitopes on target molecule and thus select antibody pairs that would work well together in your specific sandwich immunoassay.
| Antigen Options |
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| Available Antibody Pairing Formats |
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| Choice of Host | Monoclonal Antibodies: Rat Mouse Rabbit |
| Polyclonal Antibodies: Rabbit Sheep | |
| Timeline (includes recombinant protein expression) | 16-18 weeks |
| Deliverables |
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While a matched pair can be screened from existing antibody, we recommend generating antibody pairs concurrently. In this way the optimal antibody combination can be found empirically for every application. You will save time, money, and effort by generating antibody pairs together at the beginning if you can foresee the need for paired antibodies in the future. Creative Diagnostics works closely with customers to deliver satisfaction in any application.
