Polyclonal Anti-idiotypic Antibody Production

Creative Diagnostics has extensive experience in development a successful immunization protocol improving the generation of polyclonal anti-idiotype antibodies (anti-IDs). Polyclonal antibodies are a mixture of immunoglobulins that recognize different epitopes on a specific antigen. In addition to the traditional mouse and rabbit options, we also offer goat, chicken, sheep, donkey, Guinea pig, Llama, Camel, horse, dogs, bovine, primate or host animals specified by the clients.

Polyclonal Anti-idiotypic Antibody Production

Features and Benefits of Polyclonal Antibodies

  • Recognition of many epitopes on any single antigen
  • Easier detection of denatured proteins
  • Enhanced target protein signal amplification
  • Better management of antigen modifications
  • Fragmentation and purification of your antibody to obtain F(ab) & F(ab)2 fragment

Our polyclonal antibody services packages include:

Item Basic Package Peptide Package Fab & F(ab)2 Package
Experimental Scheme Design (1-2 days)
Client-Provided Antigens
  • Immunization protocol in desired host species
Peptide Synthesis (up to 20 aa) & Conjugation (3-4 weeks)
  • 5-10mg, purity > 90% by HPLC
  • Conjugation of peptide to carrier protein (KLH, BSA, OVA)
  • Immunization of conjugate into desired host species
Fab & F(ab)2 Fragmentation and purification (3-4 weeks)
  • Fab or F(ab)fragment generation with papain /pepsin
  • Fab or F(ab)purification from protein A /G
Services 1: 1 or 2 Rabbit, 63-days
  • 3-5 immunizations
  • Test bleed day 35 & ELISA titer determination
  • Final bleed: 40 – 90 ml serum
Services 2: 1 Super-Fast rabbit, 28-days
  • 4 immunizations
  • Final bleed: 40 – 90 ml serum
Services 3: PhosphoSpecific Antibodies
  • Peptide synthesis: phospho & non-phosphopeptide
  • Immunization of 2 rabbits
  • Purification: affinity purification & depletion
Antigen Affinity Purification
  • Antigens are covalently bound to agarose beads bysulfhydryls
  • Immuno-affinity purification of polyclonal antibodies

Common Applications of Polyclonal Anti-Idiotypic Antibodies

  • Preclinical research of therapeutic antibodies
  • Anti-drug Antibodies (ADA) for clinical development
  • Pharmacokinetic (PK) studies
  • Immune Response (IR) immunogenicity assays
  • Controls in ligand binding neutralizing assays
  • Controls in antibody blocking assays

The FDA recommends that the sponsor develops polyclonal anti-idiotypic antibodies as positive controls for antibody immunogenicity assays. Creative Diagnostics has a proven track record of achieving successful outcomes with higher complexity projects. Our ability to work closely with customers to clearly understand their end application, define their specific project goals and design a detailed project plan is the key to our high success rate delivering quality polyclonal anti-idiotype antibodies.

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Creative Diagnostics provides the highest quality products and services.

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