Creative Diagnostics has extensive experience in producing monoclonal antibodies in hamsters. The use of hamster as the host animal offers a unique species alternative in areas where mouse and rat antibodies may not work well. We can employ up to 10 hamsters for each antigen. Immune responses are elicited using immunogens such as transfected cell lines, DNA, recombinant proteins, native proteins, peptides or haptens conjugated to carrier molecules.
Fig.1 Outline of monoclonal antibody development in Hamster
We perform splenocyte fusion on one or more animals. We select and subclone the strongly positive clones from the parental cultures by 2 rounds of limiting dilution cloning. Stable clones will be expanded into 24-well plates and then into T-flasks for further characterization. At no additional cost, client could select one clone for in vitro production of approximately 1L of supernatant. Supernatants will be purified using Protein A/G column and pure antibodies will be deliverable. We also offer a full range of contract manufacturing services, including In vitro Antibody and Protein Production in bioreactors and large-scale Antibody Purification.