Human sCD152/CTLA-4 ELISA Kit

Sample

Serum, Plasma-EDTA, Plasma-Heparin, Amniotic fluid, Cell culture supernatant

Species Reactivity

Human

Detection Method

sELISA

Intended Use

The human sCTLA-4 ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human sCTLA-4. The human sCTLA-4 ELISA is for research use only. Not for diagnostic or therapeutic procedures.

Contents of Kit

1 vial (70 μl) Biotin-Conjugate anti-human sCTLA-4 monoclonal antibody
1 vial (150 μl) Streptavidin-HRP
2 vials human sCTLA-4 Standard lyophilized, 20 ng/ml upon reconstitution
1 vial (12 ml) Sample Diluent
1 vial (5 ml) Assay Buffer Concentrate 20× (PBS with 1% Tween 20 and 10% BSA)
1 bottle (50 ml) Wash Buffer Concentrate 20× (PBS with 1% Tween 20)
1 vial (15 ml) Substrate Solution (tetramethyl-benzidine)
1 vial (15 ml) Stop Solution (1M Phosphoric acid)

Storage

Store the complete kit at 2-8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).

Detection Range

0.16-10 ng/ml

Detection Limit

0.13 ng/ml

Sensitivity

The limit of detection of human sCTLA-4 defined as the analyte concentration resulting in an absorbance significantly higher than that of the dilution medium (mean plus 2 standard deviations) was determined to be 0.13 ng/ml (mean of 6 independent assays).

General Description

Cytotoxic T lymphocyte associated gene –4 (CTLA-4) was initially described as a classical type I glycoprotein on the surface of activated T-cells. CTLA-4 is a member of the Ig gene superfamily and along with its homologue, CD28, is a B7 binding protein. There is evidence that CTLA-4 is a negative regulator of T-cell activation, ligation of CTLA-4 on the T-cell surface initiates a series of biochemical events that attenuate an ongoing immune response . CTLA-4 knock out mice demonstrate profound polyclonal lymphoproliferative disoders that infiltrate most major organ systems and the animals die a few weeks after birth. The animals have increased levels of IgG which illustrates the role of CTLA-4 on humoral immune responses as well. A role for CTLA-4 in autoimmune disease is suggested by the observations that blockade of B7/CTLA-4 interaction exacerbates animal models of autoimmune disease such as experimental autoimmune encephalomyelitis and diabetes. A search for genetic markers that segregate with Graves' disease revealed an association with CTLA-4 polymorphisms. CTLA-4 is further more closely linked to autoimmune thyroid disease (ATD). A native soluble form of CTLA-4 has been described to be present in human serum . A correlation of circulating CTLA-4 and ATD has been shown.

Standard Curve