GeneralInformation
Immunogen
The extracellular portion of of murine CTLA-4
Fusion Species
Hamster X Mouse Heterohybridoma
Immunological Donor
Hamster spleen
Cell Line Description
The hybridoma cell line was produced by fusing Sp2/0-Ag14 myeloma cells with spleen cells from an Armenian hamster that had been immunized with the extracellular portion of of murine CTLA-4 fused to a murine IgG2a. Under conditions of suboptimal T cell receptor (TCR) stimulation, whole antibody or Fab fragments from 4F10 can augment T cell proliferation in allogeneic mixed lymphocyte reactions. However, when optimal TCR stimulation is present, 4F10 can suppress T cell proliferation.
Application Notes
Detecting and quantitating CTLA-4 expression by flow cytometric analysis, immunoprecipitating the antigen from CTLA-4 positive cells.
Growth Properties
Suspension
CultureMethod
Subculturing
Incubate cells at 37°C with 5% CO2 in air atmosphere, renew medium every 2-3 days, start cells at 2x10^5 cells/mL and maintain cultures between 1x10^5-1x10^6 cells/ml
Storage
Liquid nitrogen vapor phase.
Freezing medium: to complete growth medium, add 5%(v/v) DMSO
Target
Synonyms
CTLA4; cytotoxic T-lymphocyte-associated protein 4; cytotoxic T-lymphocyte protein 4; CD152 antigen; cytotoxic T-lymphocyte-associated antigen 4; Cd152; Ly-56; Ctla-4;
Citations
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Wang, WS; Yu, DH; et al. Biomarkers on melanoma patient T Cells associated with ipilimumab treatment. JOURNAL OF TRANSLATIONAL MEDICINE 10:-(2012).
Inoue, N; Watanabe, M; et al. Associations Between Autoimmune Thyroid Disease Prognosis and Functional Polymorphisms of Susceptibility Genes, CTLA4, PTPN22, CD40, FCRL3, and ZFAT, Previously Revealed in Genome-wide Association Studies. JOURNAL OF CLINICAL IMMUNOLOGY 32:1243-1252(2012).
COA
Certificate of Analysis
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