Coronavirus Antibodies

Our antibodies serve mainly as research tools. Coronavirus antigen proteins detection and quantification through antibodies support scientific research on immune responses to the virus and enable the development of diagnostic kits and vaccines.

The recommended storage temperatures for antibodies are usually -20°C or 4°C and avoiding multiple freeze-thaw cycles ensures their activity and specificity remain intact. Despite antibodies not being typically biohazardous substances standard biosafety procedures should be implemented during handling to avoid contamination and accidental exposure.

To determine antibody specificityscientists must conduct cross-reactivity tests which compare antibody bindingto related viruses like SARS-CoV-1, MERS-CoV and cold coronaviruses toeliminate non-specific interactions. Western blot experiments require confirmationthat the observed band corresponds to the anticipated molecular weight of theintended protein. Under suitable conditions both knockdown and knockout of thetarget protein are possible to generate cell lines that serve as negativecontrols to validate antibody specificity.

You should confirm the following parameters:

• Detection target (such as N protein, S1 subunit, or RBD)
• Sample type compatibility (serum, cell supernatant, or tissue lysate)
• Whether the detection range covers the expected concentration
• Whether a standard curve calibration is provided

The experimental applicability of antibodies depends on their epitope recognition characteristics and host species. Antibodies targeting linear epitopes (like those for the C-terminal peptide of N protein) are more suitable for Western Blot, while conformational epitope antibodies (such as those recognizing S protein trimers) are better for flow cytometry and live cell imaging. Cross-species reactivity needs to be confirmed through validation, for example, rabbit-derived antibodies used on mouse tissues require species absorption treatment.