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SARS-CoV-2 Accessory Proteins and Antibodies

Creative Diagnostics now can provide SARS-CoV-2 Accessory Protein antigens and antibodies that can be used for scientific research. Our antigens and antibodies are produced using a standardized production process to ensure the highest quality.

Recombinant SARS-CoV-2 Accessory Protein

Anti-SARS-CoV-2 Accessory Protein Antibodies

Cat. Product Name Host Application
CABT-CS068 SARS-CoV-2 ORF8 Polyclonal antibody Rabbit ELISA, WB
CABT-CS069 SARS-CoV-2 ORF3a Polyclonal antibody Rabbit ELISA, WB
CABT-CS070 SARS-CoV-2 ORF7a Polyclonal antibody Rabbit ELISA, WB
CABT-CS071 SARS-CoV-2 ORF6 Polyclonal antibody Rabbit ELISA, WB
CABT-CS072 SARS-CoV-2 ORF10 Polyclonal antibody Rabbit ELISA, WB
CABT-CS073 SARS-CoV-2 pp1ab Polyclonal antibody Rabbit ELISA, WB
CABT-CS086 SARS-CoV-2 Envelope Polyclonal antibody Rabbit ELISA
CABT-CS150 SARS-CoV-2 ORF8 (IN) Polyclonal antibody Rabbit ELISA, WB
CABT-CS151 SARS-CoV-2 ORF8 (CT) Polyclonal antibody Rabbit ELISA, WB

The SARS-COV-2 genome comprises 29,903 nucleotides, with 10 Open Reading Frames (ORFs). The 5’ terminal ORF1ab more than two-thirds of the genome encodes two viral replicase polyproteins pp1a and pp1b. The proteolytic cleavage of pp1a and pp1b produces 16 nonstructural proteins (nsp1 to nsp16), while the 3’ terminal regions encode structural viral proteins including surface (S), envelope (E), membrane (M), and nucleocapsid (N) proteins and interspersed among them the ORFs encoding nonstructural and accessory proteins 3a, 3b, 6, 7a, 7b, 8 (or 8a and 8b in some isolates) and 10. (Fig. 1)

Fig. 1: SARS-CoV-2 Complete Genome
(Source: Genomic characterization of a novel SARS-CoV-2)

Most ORFs annotated from SARS-CoV-2 were found to be conserved in other viruses, except for ORF8 and ORF10.The protein sequence of SARS-CoV-2 ORF8 shared very low similarity with those sequences in SARS-CoV and BM48-31, and ORF10 had a premature stop codon in both SARS-CoV and BM48-31.

Coronavirus accessory proteins are generally considered to be dispensable for virus replication in vitro. However, several accessory proteins have been shown to exhibit functions in virus-host interactions during coronavirus infection in vivo.

Recent studies have suggested that SARS-CoV accessory proteins may confer biological advantages to the virus in the natural host, and contribute to the pathogenesis of SARS. Extensive functional studies in cell culture and animal models have shown that SARS-CoV accessory proteins are involved in a wide variety of cellular processes, such as cell proliferation, programmed cell death, activation of stress response pathways and cytokine production. Notably, some coronaviral accessory proteins have also been shown to modulate the interferon signaling, which is of paramount importance for host antiviral immunity.

Accessory proteins also play a role in immune evasion and inflammation, including inhibition of type I interferons (ORF3b and 6), induction of apoptosis (ORF3a, 3b, 8a), modulation of cellular DNA synthesis (ORF6, 8b), induction of arms of unfolded protein response (ORF8), stimulation of chemokine synthesis (3a, stimulates chemokine ligand 5 and C-X-C motif chemokine ligand 8), and inflammation (ORF7, activates inflammation via NF-kB and MAPK-8).

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