HPV68 Pseudovirus are used to test the ability of serum and antibodies to neutralize the infectivity of HPV68. HPV68 Pseudovirus are produced in 293FT cells using three separate plasmids, encoding the HPV L1 protein, the HPV L2 protein, and a reporter gene. HPV68 Pseudovirus display antigenically correct L1 and L2 protein pseudotyped on replication-incompetent virus particles. Pseudovirus are capable of a single round of infection and carry a plasmid that expresses GFP optical reporter gene upon infection.
Nature
Virus
Application Notes
The recommended amount of pseudovirus per well can be found in the Certificate of Analysis for each lot of pseudovirus.
Storage
Store at -80°C. Multiple freeze/thaw cycles not recommended. When using the virus, transfer the virus from the -80 ° C refrigerator and melt it in an ice bath.
Ship
Frozen on dry ice
Citations
Publication ()
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