Human Papilloma Virus IgG ELISA Kit (DEIA-F678S)

Regulatory status: For research use only, not for use in diagnostic procedures.

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plasma, serum
Species Reactivity
Intended Use
Enzyme ImmunoAssay (ELISA) for determination of IgG class antibodies to Human Papilloma Virus (or HPV) in human plasma and sera.

The product is supplied for research purpose only. It is not for use in the diagnosis or for the follow-up of patients administered with the vaccines containing HPV antigens.
Contents of Kit
1. Microplate
2. Negative Control
3. Positive Control
4. Wash buffer concentrate
5. Enzyme Conjugate
6. Chromogen/Substrate
7. Sulphuric Acid
8. Specimen Diluent
For more detailed information, please download the following document on our website.


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Attendance at early recall and colposcopy in routine cervical screening with human papillomavirus testing


Authors: Green, Leonardo I.; Mathews, Christopher S.; Waller, Jo; Kitchener, Henry; Rebolj, Matejka

Attendance at early recall and colposcopy is crucial to attaining the benefits of primary high-risk human papillomavirus (HR-HPV)-based screening. Within the English HPV pilot, we analysed deprivation- and age-related patterns of attendance at colposcopy and 12- and 24-month early recall of HR-HPV positive women screened in 2013 to 2015 (N = 36 466). We fitted logistic regression models for adjusted odds ratios (OR). Despite high overall attendance, area deprivation had a small but significant impact at both early recalls, for example, attendance at 24 months was 86.3% and 83.0% in less vs more deprived areas, respectively (ORadj: 0.76; 95% CI: 0.67-0.87). Older women (>= 30 years) were more likely to attend early recall than younger women (<30 years), for example, attendance at 24 months was 86.1% vs 82.3%, respectively (ORadj: 1.32, 95% CI: 1.16-1.51). Most women attended colposcopy following a baseline referral, with 96.9% attendance among more deprived and 97.8% among less deprived areas (ORadj: 0.70; 95% CI: 0.55-0.88). Differences in colposcopy attendance by deprivation level at 12 and 24 months were of approximately the same magnitude. In conclusion, attendance at early recall and colposcopy was reassuringly high. Although there were statistically significant differences by deprivation and age group, these were small in absolute terms.

ATP synthase modulation leads to an increase of spare respiratory capacity in HPV associated cancers


Authors: Kirschberg, Matthias; Heuser, Sandra; Marcuzzi, Gian Paolo; Hufbauer, Martin; Seeger, Jens Michael; Dukic, Anamaria; Tomaic, Vjekoslav; Majewski, Slawomir; Wagner, Steffen; Wittekindt, Claus; Wuerdemann, Nora; Klussmann, Jens Peter; Quaas, Alexander; Kashkar, Hamid; Akguel, Baki

Mucosal and skin cancers are associated with infections by human papillomaviruses (HPV). The manner how viral oncoproteins hijack the host cell metabolism to meet their own energy demands and how this may contribute to tumorigenesis is poorly understood. We now show that the HPV oncoprotein E7 of HPV8, HPV11 and HPV16 directly interact with the beta subunit of the mitochondrial ATP-synthase (ATP5B), which may therefore represent a conserved feature across different HPV genera. By measuring both glycolytic and mitochondrial activity we observed that the association of E7 with ATP5B was accompanied by reduction of glycolytic activity. Interestingly, there was a drastic increase in spare mitochondrial respiratory capacity in HPV8-E7 and an even more profound increase in HPV16-E7 expressing cells. In addition, we could show that ATP5B levels were unchanged in betaHPV positive skin cancers. However, comparing HPV-positive and HPV-negative oropharyngeal squamous cell carcinomas (OPSCC) we noticed that, while ATP5B expression levels did not correlate with patient overall survival in HPV-negative OPSCC, there was a strong correlation within the HPV16-positive OPSCC patient group. These novel findings provide evidence that HPV targets the host cell energy metabolism important for viral life cycle and HPV-mediated tumorigenesis.

Giacomet, V., Penagini, F., Trabattoni, D., Viganò, A., Rainone, V., Bernazzani, G., ... & Zuccotti, G. V. (2014). Safety and immunogenicity of a quadrivalent human papillomavirus vaccine in HIV-infected and HIV-negative adolescents and young adults. Vaccine, 32(43), 5657-5661.

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