Pseudotyped GFP HPV35

Name: Pseudotyped GFP HPV35
SKU: PSVG-HPV35
Rating: 5 (1 reviews)

COA

SDS

Datasheet

Summary

Product Overview

HPV35 Pseudovirus are used to test the ability of serum and antibodies to neutralize the infectivity of HPV35. HPV35 Pseudovirus are produced in 293FT cells using three separate plasmids, encoding the HPV L1 protein, the HPV L2 protein, and a reporter gene. HPV35 Pseudovirus display antigenically correct L1 and L2 protein pseudotyped on replication-incompetent virus particles. Pseudovirus are capable of a single round of infection and carry a plasmid that expresses GFP optical reporter gene upon infection.

Nature

Virus

Application Notes

The recommended amount of pseudovirus per well can be found in the Certificate of Analysis for each lot of pseudovirus.

Storage

Store at -80°C. Multiple freeze/thaw cycles not recommended.
When using the virus, transfer the virus from the -80 ° C refrigerator and melt it in an ice bath.

Ship

Frozen on dry ice

Citations

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Background

Papillomaviruses are a group of epithelial viruses capable of infecting the skin and mucous membranes of humans and higher vertebrates, and can induce warty growths and even lead to benign and malignant tumors in the epithelial tissues. Human papillomavirus (HPV) belongs to the genus Papillomavirus in the family Papillomaviridae. More than 200 subtypes of HPV have been identified, which can be classified into high-risk and low-risk HPV according to their carcinogenicity, and high-risk HPV is the main pathogen that induces cervical cancer in women.

The HPV gene is composed of a closed-loop double-stranded DNA molecule, with slight differences in gene length between types. The HPV genome contains 8 open reading frames (ORFs) and all are on the same DNA strand. The genome can be divided into 3 sections based on functional differences. The early region (ER) encodes mainly non-structural proteins involved in replication, transcription, regulation and induction of transformation of the viral genome in the host cell. Expression of all these genes occurs prior to replication of the viral genome. The late region (LR) is responsible for encoding two important capsid proteins, but expression is restricted to proliferating infected cells. The remaining region is the more variable region, which does not contain ORFs, and consists of the long control region (LCR), the upstream regulatory region (URR), and the non-coding region, which is the site of the initiation of DNA replication and the location of important transcriptional regulatory elements.

Life cycle of high-risk HPV in epithelial tissue Figure 1. Life cycle of carcinogenic HPVs in stratified squamous epithelia
(Source: Nelson CW, et al. 2023)

HPV prophylactic vaccines are one of the most effective means of preventing HPV transmission. Currently available vaccines include bivalent, quadrivalent and nonavalent HPV vaccines. Despite the proven safety and efficacy of these vaccines, they still face many problems, one being that the sustained protective effect of HPV vaccines is still uncertain, and the other being the limited scope of HPV vaccine protection. There are so many subtypes of HPV that vaccine development for just a few HPVs will not protect patients against all high-risk HPV infections, and although some studies have now shown that there is cross-protection between some similar types of HPV itself, this effect is very weak. HPV35 is one of the high-risk HPV types strongly associated with cervical cancer, but is not the most common high-risk HPV subtype. HPV35 is most closely related to HPV16 and HPV31, and most regions are conservatively similar, making it suitable for study as a crossover vaccine.

Alternative Names

Pseudotyped GFP Human papillomavirus 35
Pseudotyped GFP Human papillomavirus type 35

References

1. Nelson CW, et al. Human papillomavirus genomics: Understanding carcinogenicity. Tumour Virus Res. 2023 Jun;15:200258.
2. Mlynarczyk-Bonikowska B, et al. HPV Infections-Classification, Pathogenesis, and Potential New Therapies. Int J Mol Sci. 2024 Jul 11;25(14):7616.

Documents

pdf COA

pdf SDS

pdf Datasheet

Pseudotyped Reporter Viruses

HPV Reagents for Vaccine Research and Immunoassay Development

QA & Reviews

Q & A

Ask a Question

Q: Can you please help me understand the safety risk of a pseudo-virus and how it might compare to the safety level of a non-replicative lentivirus or retrovirus?

A: Because PsV are capable of transferring foreign DNA, they should be handled using full biosafety level 2 precautions. It is important to note that the promiscuity of packaging by L1 and L2 can lead to generation of PsV with encapsidated fragments of cellular DNA, possibly including SV40 large tumor antigen, adenovirus oncogenes, or unknown oncogenes present in 293TT cells. Mature PsV can be inactivated by 70% ethanol, but, like many other types of non-enveloped virus particles, they are resistant to a wide spectrum of physical insults, including various detergents and proteases, prolonged heating at 56°C, 50 mM EDTA, and sodium chloride up to at least 1.5 M (unpublished results). PsV are probably also resistant to dessication. High-titer PsV should never be harvested using tip sonication, which can create potentially dangerous aerosols.

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