Pseudotyped GFP HPV16

Name: Pseudotyped GFP HPV16
SKU: PSVG-HPV16
Rating: 5 (1 reviews)

COA

SDS

Datasheet

Summary

Product Overview

HPV16 Pseudovirus are used to test the ability of serum and antibodies to neutralize the infectivity of HPV16. HPV16 Pseudovirus are produced in 293FT cells using three separate plasmids, encoding the HPV L1 protein, the HPV L2 protein, and a reporter gene. HPV16 Pseudovirus display antigenically correct L1 and L2 protein pseudotyped on replication-incompetent virus particles. Pseudovirus are capable of a single round of infection and carry a plasmid that expresses GFP optical reporter gene upon infection.

Nature

Virus

Application Notes

The recommended amount of pseudovirus per well can be found in the Certificate of Analysis for each lot of pseudovirus.

Storage

Store at -80°C. Multiple freeze/thaw cycles not recommended.
When using the virus, transfer the virus from the -80 ° C refrigerator and melt it in an ice bath.

Ship

Frozen on dry ice

Citations

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Documents

pdf COA

pdf SDS

pdf Datasheet

Pseudotyped Reporter Viruses

HPV Reagents for Vaccine Research and Immunoassay Development

QA & Reviews

Q & A

Ask a Question

Q: Can you please help me understand the safety risk of a pseudo-virus and how it might compare to the safety level of a non-replicative lentivirus or retrovirus?

A: Because PsV are capable of transferring foreign DNA, they should be handled using full biosafety level 2 precautions. It is important to note that the promiscuity of packaging by L1 and L2 can lead to generation of PsV with encapsidated fragments of cellular DNA, possibly including SV40 large tumor antigen, adenovirus oncogenes, or unknown oncogenes present in 293TT cells. Mature PsV can be inactivated by 70% ethanol, but, like many other types of non-enveloped virus particles, they are resistant to a wide spectrum of physical insults, including various detergents and proteases, prolonged heating at 56°C, 50 mM EDTA, and sodium chloride up to at least 1.5 M (unpublished results). PsV are probably also resistant to dessication. High-titer PsV should never be harvested using tip sonication, which can create potentially dangerous aerosols.

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