HPV16 Pseudovirus are used to test the ability of serum and antibodies to neutralize the infectivity of HPV16. HPV16 Pseudovirus are produced in 293FT cells using three separate plasmids, encoding the HPV L1 protein, the HPV L2 protein, and a reporter gene. HPV16 Pseudovirus display antigenically correct L1 and L2 protein pseudotyped on replication-incompetent virus particles. Pseudovirus are capable of a single round of infection and carry a plasmid that expresses GFP optical reporter gene upon infection.
The recommended amount of pseudovirus per well can be found in the Certificate of Analysis for each lot of pseudovirus.
Store at -80°C. Multiple freeze/thaw cycles not recommended.
When using the virus, transfer the virus from the -80 ° C refrigerator and melt it in an ice bath.
Biosafety Level: BSL-2
It is the responsibility of the principal investigator to seek Institutional Biosafety Safety Committee approval for recombinant DNA, transgenic animal or infectious agent use within their laboratory spaces and maintain an Institutional Biosafety Safety Committee approval during the time period these materials are used.