Human Heparin-Platelet Factor 4 Complex Antibody (IgG) ELISA Kit

Sample

Serum, plasma, tissue homogenates and other biological fluids.

Species Reactivity

Universal

Detection Method

cELISA

Intended Use

Enzyme immunoassay for determination of HPF4.

Contents of Kit

1. Please store the kit at 4°C if used up in 1 week. If used for more than 1 week, store the Pre-Coated Microplate, Standard, Biotinylated-Conjugate and Streptavidin-HRP at -20°C and all other reagents at 4°C according to the temperature indicated on the label. Avoid repeated freeze-thaw cycles.
2. Do not use the kit beyond the expiration date.
3. After opening the package, please check that all components are complete.
4. The cap must be tightened to prevent evaporation and microbial contamination. The reagents volume is slightly more than the volume marked on labels, please use accurate measuring equipment and do not pour directly into the vial.
All kit components have been formulated and quality control tested to function successfully. Do not mix or substitute reagents or materials from other kits, detection effect of the kit will not be guaranteed if utilized separately or substituted.

Storage

Store the kit at 4°C for 1 week. If the kit is not used up in 1 week, store the items separately according to the following conditions after the kit is received.

Precision

Intra-assay Precision (Precision within an assay):CV% < 8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays):CV% < 10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Detection Range

1.57-100 ng/mL

Sensitivity

0.59 ng/mL