Sample
cell culture supernatants, serum, platelet-poor plasma
Detection Method
Sandwich ELISA
Intended Use
For the quantitative determination of mouse Platelet Factor 4 (PF4) concentrations in cell culture supernates, mouse serum, and platelet-poor plasma.
Contents of Kit
1. Mouse PF4 Microplate
2. Mouse PF4 Standard
3. Mouse PF4 Control
4. Mouse PF4 Conjugate
5. Assay Diluent
6. Calibrator Diluent Concentrate
7. Wash Buffer Concentrate(25X)
8. Color Reagent A
9. Color Reagent B
10. Stop Solution
Storage
Store at 4°C for frequent use, at -20°C for infrequent use. Avoid multiple freeze-thaw cycles.
Return unused wells to the foil pouch containing the desiccant pack, reseal along entire edge of zip-seal. May be stored for up to 1 month at 2-8°C.
Precision
Intra-assay Precision:
3.0-7.1%
Inter-assay Precision:
7.5-7.8%
Detection Range
0.003-0.010 ng/mL
Citations
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Sitter, T; Spannagl, M; et al. Prevalence of heparin-induced PF4-heparin antibodies in hemodialysis patients. NEPHRON 79:245-246(1998).
Hassan, S; Qureshi, W; et al. Heparin-induced thrombocytopenia: is it a graft-threatening complication?. TRANSPLANT INTERNATIONAL 26:385-391(2013).
COA
Certificate of Analysis
