Intended Use
For Research Use Only. For the quantitative and qualitative determination of IgA,IgG,IgM antibodies against double-stranded DNA (dsDNA) in human serum.
Contents of Kit
1. Sample Buffer (5×), 1 × 20mL,
2. Wash Buffer (50×), 1 × 20mL
3. Negative Control, 1 × 1.5mL
4. Positive Control, 1 × 1.5mL
5. Cut-off Calibrator, 1 × 1.5mL
6. Calibrators, 6 × 1.5mL, Concentration of each calibrator: 0, 3, 10, 30, 100, 300 U/mL.
7. Conjugate IgA/G/M, 1 × 15mL
8. TMB Substrate, 1 × 15mL
9. Stop Solution, 1 × 15mL, 1M Hydrochloric Acid
10. Microtiter plate strips, 12 × 8 well
Storage
Store all reagents and the microplate at 2-8°C/35-46°F, in their original containers. Reagents and the microplate must be used within the expiry date indicated on each component, only. Avoid intense exposure of TMB solution to light. Store microplates in designated foil, including the desiccant, and seal tightly.
Detection Range
0-300 U/mL
General Description
Anti-double stranded DNA (Anti-dsDNA) antibodies are a group of anti-nuclear antibodies (ANA) the target antigen of which is double stranded DNA. Blood tests such as enzyme-linked immunosorbent assay (ELISA) and immunofluorescence are routinely performed to detect anti-dsDNA antibodies in diagnostic laboratories. They are highly diagnostic of systemic lupus erythematosus (SLE) and are implicated in the pathogenesis of lupus nephritis.
Citations
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