Specificity: Recognizes double stranded DNA expressed in the nucleus of eukaryotes.
Species Reactivity: all eukaryotes
Application: FC, IF, IHC
Summary
Specifications
Antibody Isotype
IgG3k
Clone
BF3
Species Reactivity
all eukaryotes
Immunogen
Nuclei of Raji Burkitt's lymphoma cells
Conjugate
Unconjugated
Applications
Application Notes
FC (1-2 μg/million cells in 0.1 ml, fix cells in 4% PFA for 10 min, at 4°C, permeabilize with 0.2% saponin or digitonin for 15 min, at 4°C) IF (1-2 μg/ml) IHC (1-2 μg/ml for 30 min at RT; staining of formalin-fixed tissues requires incubating tissue sections in 4N HCl, for 30 minutes)
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