Anti-dsDNA (Mouse) IgM ELISA Kit

Sample

Serum, plasma

Species Reactivity

Mouse

Intended Use

The ELISA Kit is designed for quantitative and rapid measurement of mouse anti-dsDNA IgM antibody levels in serum or plasma samples.

Storage

All the kit components should be stored at -20°C and the microtiter plate should be kept in a sealed bag with desiccant to minimize
exposure to damp air.

Precision

Standard was added with known amounts of anti-dsDNA IgM antibodies and then diluted with Sample/Standard/Secondary Antibody Dilution Buffer to assay anti-dsDNA IgM antibodies by ELISA.

Detection Range

0.8 ng/mL to 50 ng/mL

General Description

High levels of serum autoantibodies against deoxyribonucleic acid (DNA) are observed in most patients with systemic lupus erythematosus (SLE) (1, 2), therefore the presence of anti-DNA antibodies in serum is considered a valuable marker for the diagnosis of SLE. Moreover, the serum anti-DNA antibodies form anti-DNA/DNA immune complexes which play an important role in the immunopathogenesis of lupus nephritis caused by SLE (3). Anti-single stranded DNA (ssDNA) IgG antibodies are elicited in the early stages of SLE, whereas anti-double stranded DNA (dsDNA) IgG antibody levels correlate with the severity of SLE. On the other hand, anti-dsDNA lgM antibodies are not specific to SLE, but correlate with the prognosis of lupus nephritis in patients with SLE (4, 5). Therefore, evaluating immunoglobulin levels of different isotypes against individual DNA types may signify a particular stage and the prognosis of SLE.
Mouse SLE models, which translate relevant information to the human condition, elucidate the cellular and genetic requirements. For example, in spontaneous mouse NZB/W F1 lupus models, anti-dsDNA antibody isotype class switching from lgM to IgG indicates renal failure which is observed in human SLE (6). Nonetheless, in artificial pristane-induced Balb/c lupus models, anti-ssDNA lgM antibodies solely induce SLE (7-9). Therefore, to study the diverse roles of anti-DNA antibodies in these mouse SLE models, Creative Diagnostics, Inc. provides anti-dsDNA lgM (Catalog # DEIA-PY07130) antibody ELISA kits.