Synthetic polynucleotide homopolymers [poly(dA-dT)-poly(dA-dT) and poly(dI-dC)-poly(dI-dC)]
Fusion Species
Mouse X Mouse Hybridoma
Immunological Donor
Mouse spleen
Myeloma
FO
Clone
DI37-2463
Cell Line Description
Animals were immunized with synthetic polynucleotide homopolymers [poly(dA-dT)-poly(dA-dT) and poly(dI-dC)-poly(dI-dC)] then boosted with foreign native DNA (EcoR1 digested pBR322 DNA). Spleen cells were fused with FO myeloma cells.
Morphology
Lymphoblast
Growth Properties
Suspension
CultureMethod
Subculturing
Incubate cells at 37°C with 5% CO2 in air atmosphere, renew medium every 2-3 days, start cells at 2x10^5 cells/mL and maintain cultures between 1x10^5-1x10^6 cells/ml
Storage
Liquid nitrogen vapor phase.
Citations
Publication ()
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