Contents of Kit
1. Sample Buffer (5×), 1 × 20mL. 5× concentrated. Tris, sodium chloride (NaCl), bovine serum albumin (BSA), sodium azide <0.1% (preservative)
2. Wash Buffer (50×), 1 × 20mL. 50× concentrated. Tris, NaCl, Tween, sodium azide <0.1 (preservative)
3. Negative Control, 1 × 1.5mL. Human serum (diluted), bovine serum, albumin (BSA), sodium azide <0.1% (preservative)
4. Positive Control, 1 × 1.5mL. Human serum (diluted), bovine serum albumin (BSA), sodium azide <0.1% (preservative)
5. Cut-off Calibrator, 1 × 1.5mL. Human serum (diluted), bovine serum albumin (BSA), sodium azide <0.1% (preservative)
6. Calibrators, 6 × 1.5mL*.Concentration of each calibrator: 0, 3, 10, 30, 100, 300 U/mL. Human serum (diluted), bovine serum albumin (BSA), sodium azide <0.1% (preservative) * Color increasing with concentration
7. Conjugate, IgM. 1 × 15mL. Containing: Anti-human Immunoglobulins conjugated to horseradish peroxidase, bovine serum albumin (BSA).
8. TMB Substrate. 1 × 15mL. Stabilized tetramethylbenzidine and hydrogen peroxide (TMB/H2O2)
9. Stop Solution. 1 × 15mL. 1M Hydrochloric Acid
10. Microtiter plate. 12 × 8 well strips. With breakaway microwells.
Storage
Store all reagents and the microplate at 2-8°C/35-46°F, in their original containers. Once prepared, reconstituted solutions are stable at 2-8°C/35-46°F for 1 month. Reagents and the microplate shall be used within the expiry date indicated on each component, only. Avoid intense exposure of TMB solution to light. Store microplates in designated foil, including the desiccant, and seal tightly.
COA
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