Intended Use
For the quantitative and qualitative determination of IgA antibodies against double-stranded DNA (dsDNA) in human serum
Contents of Kit
1. Sample Buffer (5×), 1×20ml
2. Wash Buffer (50×), 1×20ml
3. Negative Control, 1×1.5ml
4. Positive Control, 1×1.5ml
5. Cut-off Calibrator, 1×1.5ml
6. Calibrators, 6×1.5ml. 0, 3, 10, 30, 100, 300 U/ml
7. Conjugate IgA, 1×15ml
8. TMB Substrate, 1×15ml
9. Stop Solution, 1×15ml
10. 12×8 well Microtiter plate strips
Storage
Store all reagents and the microplate at 2-8°C/35-46°F, in their original containers. Once prepared, reconstituted solutions are stable at 2-8°C/35-46°F for 1 month. Reagents and the microplate shall be used within the expiry date indicated on each component, only. Avoid intense exposure of TMB solution to light. Store microplates in designated foil, including the desiccant, and seal tightly.
Detection Range
0-300 U/ml
General Description
Anti-ds-DNA-A ELISA is a solid phase enzyme immunoassay with human recombinant double-stranded DNA (dsDNA) for the quantitative and qualitative detection of IgA antibodies against dsDNA in human serum.
Anti-dsDNA antibodies mainly recognize the phosphate units of the DNA; thus these autoantibodies also bind single stranded DNA (ssDNA). To ensure correct quantitation of anti-dsDNA antibodies the used antigen has been proven to be free of contamination with ssDNA.
Citations
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