Intended Use
This kit is designed for detecting and quantitatively analyzing mouse anti-dsDNA antibody.
For research use only. Not for use in diagnostic procedures.
Contents of Kit
1.96-Well 12 strip Plate coated with ds-DNA, 1
2.Anti-mouse IgG conjugated to HRP, 10μL
3.dsDNA mouse IgG standard 25 μg/ml, 10μL
4.1X Diluent buffer, 40mL
5.5X Assay wash buffer, 40mL
6.Substrate, 10mL
7.Stop solution, 5mL
Storage
Store all reagents at 2-8°C.
All reagents must be brought to room temperature (20- 25°C) prior to use.
When stored at 2-8°C, the diluted Assay wash buffer is stable until the kit expiration date.
Detection Range
4 ng/ml to 125 ng/ml
General Description
Anti-dsDNA antibodies that appear to be critical in the pathogenesis of tissue injury are characteristic of systemic lupus erythematosus (SLE). There is a good correlation between anti-dsDNA antibody levels and disease activity. The overall detection rate of these antibodies is approximately 50-55% in SLE patients and about 89% in SLE patients with active renal disease. When they are present in high concentration, anti-dsDNA antibodies are virtually specific for SLE (>90%). Antibodies to dsDNA may disappear with immunosuppressive treatment and during remission. They rarely occur in other autoimmune disorders. Creative Diagnostics has developed anti-dsDNA ELISA, a sandwich quantitative assay, to screen the presence of serum ds-DNA antibodies IgG.
Standard Curve
This Standard curve is for demonstrative purpose only.
A standard curve must be run with each assay.
Citations
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