Anti-Aflatoxin monoclonal antibody, clone CVD

Staphylococcus aureus (S. aureus) causes serious food-borne diseases, and tools able to directly profile intact S. aureus would greatly facilitate food safety and public health. Herein, we proposed a biosensing platform for culture-independent and separation-free profiling S. aureus, thus allow us to directly detect intact S. aureus in complex samples. The binding protection effect of aptamer-cell complex was introduced to construct the aptasensor, and it allowed to eliminate the optimization of aptamer probe sequences. The proposed aptasensor, terms enzymatic cleavage aptasensor could achieve a sensitive (a detection limit of 64 CFU/mL) and broad-concentration quantification (dynamic range 10(2)-10(7) CFU/mL) of S. aureus. Furthermore, it could specifically identify intact S. aureus in complex samples, and the quantifying of S. aureus was achieved in tap water, milk and porker with high precision. Therefore, enzymatic cleavage aptasensor could be a good candidate for on-site biosensing platform of S. aureus, as well as other pathogens by replacing the aptamer sequences. (C) 2020 Elsevier B.V. All rights reserved.

This study reports the fabrication of orange peels (OP) extract (water:ethanol:acetonitrile) modified organic-inorganic hybrid bentonite (OP-bentonite). The synthesized composite was employed for the adsorptive removal of carcinogenic mycotoxins (aflatoxin B1 (AFB1), fumonisin B1(FB1) and ochratoxin (OTA)). The product was texturally characterized by BET, FTIR, XRD, TGA, SEM, EDX and XPS techniques while quantification of toxins was performed using HPLC/LC-MS. The effect of reaction temperature, pH, adsorbent dosage, coexisting mycotoxins and gastro-intestinal fluids on the adsorption performance of OP-bentonite was systematically investigated. Isothermal data (pH 2.5 and 7.0) for toxins adsorption agreed well with the Langmuir isotherm model revealing maximum adsorption capacity (q(max)) (mg/g) of 166 and 121 for AFB1, 3.87 and 1.78 for FB1 and 1.97 and 3.19 for OTA respectively. The adsorption kinetics followed pseudo-second-order kinetic model while its adsorption capacity surpassed many adsorbents reported for AFB1, FB1 and OTA with orders of magnitude. Therefore, attributed to the advantages of being efficient, green and simplified synthesis, OP-bentonite could be envisaged as a promising adsorbent for the enhanced sequestration of mycotoxins.