Aflatoxin B1 Precise Test (Edible Oil)

Aflatoxins are toxic secondary metabolites primarily produced by Aspergillus flavus and A. paraciticus. Exposure to these mycotoxins through contaminated food and feed may cause oxidative stress and liver toxicity in animals. One of the promising strategies to mitigate aflatoxin accumulation is the biological management during pre-harvest using non-aflatoxigenic A. flavus. The mechanism offered by these strains in mitigating aflatoxin is still unclear. Thus, the aim of the present study is to delineate the mechanism of intraspecific inhibition of aflatoxin production. Among the 18 non-aflatoxigenic strains evaluated, six strains were able to reduce more than 50% of the aflatoxins produced by the native aflatoxigenic strains. The non-aflatoxigenic strains used in this study failed to degrade the aflatoxins. Eventhough, the non-aflatoxigenic strains were not able to inhibit the synthesis of aflatoxins completely. Four non-aflatoxigenic isolates could competitively excluded the aflatoxigenic strain. Furthermore, when non-aflatoxigenic and an aflatoxigenic isolate were separated by 0.4 and 3 mu m filters, aflatoxin synthesis was not significantly reduced. However, when the pore size was 8 mu m, there was a significant decrease in aflatoxin production. This results suggest the role of physical contact between the hyphae, thigmoregulation, in the inhibition of aflatoxin production. Additionally, to better understand the transcriptional level control of this phenomenon, we analyzed the gene expression profile of aflatoxin biosynthesis genes in the aflatoxigenic strain. The aflatoxin biosynthesis genes were down regulated in the aflatoxigenic strain in contact with non-aflatoxigenic strain group when compared to the control. This is the first evidence of the combined action of competitive exclusion and thigmodownregulation which led to the intraspecific inhibition of aflatoxin production.

The demand of simple, sensitive, selective and reliable assay for aflatoxin B1 (AFB1) detection is ubiquitous in food safety, due to its high toxic. Herein, a novel fluorescent aptasensor using metal-organic frameworks (UiO66-NH2) and TAMRA label aptamer as sensing platform for AFB1 detection was developed. The TAMRA aptamer adsorbed on the surface of UiO-66-NH2 via van der Waals force and its fluorescence was quenched for the charge transfer from fluorescence dye TAMRA to metal ions of UiO-66-NH2. After introducing AFB1 to the system, the TAMRA aptamer binded to AFB1 and formed TAMRA aptamer/AFB1complex, making its conformation change and resulting in fluorescence recovery. Thus, the quantity of AFB1 could be analyzed according to the fluorescence signal change. Under optimize experimental conditions, the assay exhibited high sensitivity toward AFB1 in range of 0-180 ng mL(-1) with low limit of detection of 0.35 ng mL(-1) and good specificity against other toxins. Moreover, the aptamer/metal-organic frameworks sensing platform could be utilized to determine AFB1 content in food samples such as corn, rice and milk. It provided a reasonable method for other mycotoxin detection by changing the sequence of aptamer.