Aflatoxin B1 ELISA Kit (DEIA054)

Regulatory status: For research use only, not for use in diagnostic procedures.

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cereals, feed, milk, milk powder, enzyme, cookies, soy sauce, vinegar, oils, peanuts
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Intended Use
Aflatoxin B1 ELISA Test Kit is a competitive enzyme immunoassay for the quantitative analysis of Aflatoxin B1 in cereals, feed, milk, milk powder, enzyme, cookies, soy sauce, vinegar, oils, peanuts .The unique features of the kit are:
1. High recovery (75-125%), rapid(10-40minutes), cost-effective extraction methods.
2. High sensitivity (0.02 ng/g or ppb).
3. High reproducibility.
4. A quick ELISA assay (only 1 hour regardless of number of samples).
Store the kit at 2-8°C. The shelf life is 12 months when the kit is properly stored.


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Dealing with aflatoxin B(1)dihydrodiol acute effects: Impact of aflatoxin B-1-aldehyde reductase enzyme activity in poultry species tolerant to AFB(1)toxic effects


Authors: Murcia, Hansen; Diaz, Gonzalo J.

Aflatoxin B(1)aldehyde reductase (AFAR) enzyme activity has been associated to a higher resistance to the aflatoxin B-1(AFB(1)) toxicity in ethoxyquin-fed rats. However, no studies about AFAR activity and its relationship with tolerance to AFB(1)have been conducted in poultry. To determine the role of AFAR in poultry tolerance, the hepaticin vitroenzymatic activity of AFAR was investigated in liver cytosol from four commercial poultry species (chicken, quail, turkey and duck). Specifically, the kinetic parameters V-max, K(m)and intrinsic clearance (CLint) were determined for AFB(1)dialdehyde reductase (AFB(1)-monoalcohol production) and AFB(1)monoalcohol reductase (AFB(1)-dialcohol production). In all cases, AFB(1)monoalcohol reductase activity saturated at the highest aflatoxin B(1)dialdehyde concentration tested (66.4 mu M), whereas AFB(1)dialdehyde reductase did not. Both activities were highly and significantly correlated and therefore are most likely catalyzed by the same AFAR enzyme. However, it appears that production of the AFB(1)monoalcohol is favored over the AFB(1)dialcohol. The production of alcohols from aflatoxin dialdehyde showed the highest enzymatic efficiency (highest CL(int)value) in chickens, a species resistant to AFB(1); however, it was also high in the turkey, a species with intermediate sensitivity; further, CL(int)values were lowest in another tolerant species (quail) and in the most sensitive poultry species (the duck). These results suggest that AFAR activity is related to resistance to the acute toxic effects of AFB(1)only in chickens and ducks. Genetic selection of ducks for high AFAR activity could be a means to control aflatoxin sensitivity in this poultry species.

Determination of aflatoxin M(1)and deoxynivalenol biomarkers in infants and children urines from Bangladesh


Authors: Ali, Nurshad; Manirujjaman, M.; Rana, Sohel; Degen, Gisela H.

The mycotoxins aflatoxin B-1(AFB(1)) and deoxynivalenol (DON) are found worldwide in crops and dietary staples. The prevalence and levels of these contaminants can vary greatly, and data in Bangladeshi food commodities are scarce. To characterize human exposure, we have conducted biomonitoring, analyzing AFM(1)(a metabolite of AFB(1)) and DON levels in urines of adult cohorts in Bangladesh. Yet, AFM(1)and DON occurrence has not been studied in the very young population of this country. Thus, the same methods, HPLC-FD for AFM(1)and LC-MS/MS for DON analysis, were now applied to determine these biomarkers in urines of infants (n = 49) and young children (n = 105) in Rajshahi and Dhaka district. Overall, AFM(1)and DON detection frequency was 43.5% and 33.4%, with 34.7% and 11.5% in infant and 47.6% and 39.4% in children urines, respectively. The mean AFM(1)levels in all infants (9.1 +/- 14.3, max 55.6 pg/mL) and children (8.8 +/- 12.9, max 75.3 pg/mL) were not significantly different. The AFM(1)mean level was slightly higher in Dhaka (9.4 +/- 12.4) compared to Rajshahi (8.5 +/- 13.9 pg/mL) district. The average DON level was about 2-fold higher in infant (3.8 +/- 2.9, max 6.8 ng/mL) than children urines (1.6 +/- 1.8, max 8.6 ng/mL), and higher in Rajshahi (2.1 +/- 2.3 ng/mL) than Dhaka (1.4 +/- 1.6 ng/mL) district. The biomarker-based estimated average daily DON intake (29.6 +/- 108.3 ng/kg bw in infants and 36.4 +/- 81.8 ng/kg bw in children) or the maximum exposure (560 ng/kg bw) do not exceed the current maximum provisional tolerable daily intake value of 1 mu g/kg bw for DON, although DON exposure in infants and children is higher than that of Bangladeshi adults. The AFM(1)urine levels in young children are somewhat lower than those found previously in adult cohorts in Bangladesh, but the frequent detection of this biomarker for AFB(1)exposure raises further concerns, also for this vulnerable part of the population. Therefore, continuous surveillance for aflatoxins in Bangladeshi food commodities is clearly required, first to identify major sources of intake and then to reduce exposure.

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