Intended Use
Enzyme Immunoassay for the quantification of Aflatoxin (total) in food (extraction, dilution, centrifugation).
Contents of Kit
1. Antibody-Binding Protein coating microplate, 8 × 12 strips, 4°C
2. Standards (0, 0.05, 0.1, 0.25, 0.5, 1.5 ng/mL Aflatoxin Standards, dyed red), 1 mL each (ready to use), 4°C
3. Diluent Buffer (dyed red), 60 mL × 2 (ready to use), 4°C
4. Aflatoxin Conjugate (Aflatoxin-Peroxidase, dyed red), 6 mL (ready to use), 4°C
5. Antibody (Anti-Aflatoxin Antibody, dyed blue), 6 mL (ready to use), 4°C
6. 10× Wash Buffer, 60 mL, 4°C
7. TMB Substrate, 15 mL (ready to use), 4°C (protect from light)
8. STOP Solution, 15 mL (ready to use), 4°C
Storage
Store the kit at 2-8°C at all times.
Precision
The CV value of intra-assay was 4.7 % and inter-assay was 6.6 %.
Sensitivity
The limit of detection (LOD) of the Aflatoxin (total) ELISA kit is 0.015 ng/mL
General Description
Aflatoxins are poisonous carcinogens and mutagens that are produced by certain molds (Aspergillus flavus and Aspergillus parasiticus) which grow in soil, decaying vegetation, hay, and grains. They are regularly found in improperly stored staple commodities such as cassava, chili peppers, cottonseed, millet, peanuts, rice, sesame seeds, sorghum, sunflower seeds, sweetcorn, tree nuts, wheat, and a variety of spices. When contaminated food is processed, aflatoxins enter the general food supply where they have been found in both pet and human foods, as well as in feedstocks for agricultural animals. Animals fed contaminated food can pass aflatoxin transformation products into eggs, in the findings of high percentages of samples of aflatoxin-contaminated chicken meat and eggs in Pakistan.
Children are particularly affected by aflatoxin exposure, which is associated with stunted growth, delayed development, liver damage, and liver cancer. An association between childhood stunting and aflatoxin exposure has been reported in some studies but could not be detected in all. Furthermore, a causal relationship between childhood stunting and aflatoxin exposure has yet to be conclusively shown by epidemiological studies, though such investigations are under way. Adults have a higher tolerance to exposure, but are also at risk. No animal species is immune. Aflatoxins are among the most carcinogenic substances known. After entering the body, aflatoxins may be metabolized by the liver to a reactive epoxide intermediate or hydroxylated to become the less harmful aflatoxin M1.
Standard Curve
The following figures demonstrate typical results with the Aflatoxin (total) ELISA Kit. One should use the data below for reference only. This data should not be used to interpret actual results.
Citations
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