For quantitative analysis of the total Aflatoxins in food and feed samples. The Aflatoxin Total ELISA is a competitive enzyme immunoassay for the screening of food and feed samples on the presence of this mycotoxin. The test is based on antibodies against Total Aflatoxin. With this ELISA-kit 96 analyses can be performed. Samples and standards are measured in duplicate which means that in total 40 samples can be analysed. The ELISA kit contains all the reagents, including standards, required to perform the test. Materials and chemicals necessary for extraction or concentration steps are not included in the test-kit.
Contents of Kit
1. Dilution buffer (20 mL, Ready-to-use)
2. Rinsing buffer (30 mL, 20x concentrated)
3. Substrate solution (12 mL, Ready-to-use)
4. Stop solution (15 mL, Ready-to-use)
5. Conjugate (70 μL, 100x concentrated)
6. Antibody (100x concentrated)
7. Zero Standard (2 mL, Ready-to-use)
8. Standard solution 1 (1 mL, Ready-to-use) 0.0313 ng/mL
9. Standard solution 2 (1 mL, Ready-to-use) 0.0625 ng/mL
10. Standard solution 3 (1 mL, Ready-to-use) 0.125 ng/mL
11. Standard solution 4 (1 mL, Ready-to-use) 0.25 ng/mL
12. Standard solution 5 (1 mL, Ready-to-use) 0.5 ng/mL
13. Standard solution 6 (1 mL, Ready-to-use) 1.0 ng/mL
1. Store the kit at 2-8°C in a dark place.
2. After the expiry date (see kit label) has passed, it is no longer possible to guarantee the accuracy of the results.
3. Before opening the sealed plate, the plate should be at ambient temperature, to avoid condensation in the ELISA plate after the plate is transported from the refrigerator to room temperature.
4. Put components at ambient temperature and reconstitute or dilute the kit components before use.
5. After the lyophilised antibody has been reconstituted, this antibody can be stored in a refrigerator for maximally 4 months (stored at 2-8°C in the dark). Alternatively, after reconstitution of the antibody, aliquots of this solutions can be prepared. The aliquots can be stored in a freezer (-20°C) till the expiry date. The substrate and standard solutions can be stored in a refrigerator (2-8°C) until the expiry date stated on the label.
6. Any direct action of light on the chromogen solution should be avoided.
Degeneration of the reagents may have occurred when the following phenomena are observed:
- A blue colouring of the chromogen solution before transferring it into the wells.
- A weak or absent colour reaction of the maximum binding (zero standard) (E450nm < 0.8).
The Aflatoxins (Aflatoxins B1, B2, G1, G2) are a group of structurally related, extremely toxic compounds produced by the moulds Aspergillus flavus, A. paraciticus and A. nomius. Aflatoxins cause cancer, mainly of the liver but also of the gut, lungs and breasts. These moulds occur on food, feed and their ingredients, derived from tropical and sub-tropical areas. The most pronounced contamination has been encountered in cereals, rice, maize, soy, tree nuts and peanuts.
Within the EU, maximum levels (MLs) for a number of mycotoxins have been set by Commission Regulation (EC) 1881/2006 (which has replaced earlier Regulations (EC) No 1525/98, 194/97 and 466/2001). Concerning the Aflatoxins, this Regulation has been amended by Commission Regulation (EC) No 165/2010. In this amending Regulation, changes in the MLs were needed for alignment with Codex Alimentarius FAO/WHO standards, and new groups of foodstuffs (oilseeds and apricot kernels) are specified.