Aflatoxin B1 ELISA Kit

Name: Aflatoxin B1 ELISA Kit
SKU: DEIA6846
Rating: 5 (1 reviews)

Regulatory status: For research use only, not for use in diagnostic procedures.

COA

SDS

Datasheet

Summary

Sample

edible oil, peanut, cereal,feed (feedstuff, compound and concentrated),soy and vinegar.

Detection Method

cELISA

Intended Use

This kit can be used for qualitative and quantitative analysis of aflatoxin B1 in edible oil, peanut, cereal,feed (feedstuff, compound and concentrated),soy and vinegar.

Contents of Kit

1. Microtiter plate precoated with antigen, 96 wells
2. Standard Solution ×6 bottle (1ml/bottle), 0ppb, 0.02ppb, 0.06ppb, 0.18ppb, 0.54ppb,1.62ppb
3. Enzyme conjugate, 7ml, red cap
4. Antibody solution, 7ml, green cap
5. Substrate A, 7ml, white cap
6. Substrate B, 7ml, red cap
7. Stop solution, 7ml, yellow cap
8. 20×concentrated wash solution, 40ml, transparent cap
9. 2×concentrated extraction solution, 50ml, blue cap

Storage

Storage condition: 2-8°C.
Storage period: 12 months.

Performance Characteristics

Accuracy
Edible oil: 90±15%
Peanut: 90±15%
Cereal: 90±15%
Feed: 90±15%
Soy: 90±15%
Vinegar: 90±15%
Nut: 100±30%

Precision

Variation coefficient of the ELISA kit is less than 10%.

Detection Limit

Edible oil: 0.1ppb
Peanut: 0.2 ppb
Cereal: 0.05ppb
Feed: 2ppb
Vinegar: 2ppb
Soy: 2ppb
Nut: 5ppb

Sensitivity

0.02ppb

General Description

Aflatoxin B1 are naturally occurring mycotoxins that are produced by many species of Aspergillus, a fungus, the most notable ones being Aspergillus flavus and Aspergillus parasiticus. This product is based on indirect competitive ELISA, which is rapid, accurate and sensitive compared with conventional instrumental analysis. It needs only 45min in one operation, which can considerably reduce operation error and work intensity.

Citations

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Documents

pdf COA

pdf SDS

pdf Datasheet

QA & Reviews

Customer Reviews

References

Investigation of aflatoxins level in some packaged and bulk legumes collected from Tehran market of Iran

INTERNATIONAL JOURNAL OF ENVIRONMENTAL ANALYTICAL CHEMISTRY

Authors: Ahmadi, Mehdi; Khaniki, Gholamreza Jahed; Shariatifar, Nabi; Molaee-Aghaee, Ebrahim

Abstract

Aflatoxins are types of mycotoxins that can cause acute and chronic toxicity in human. In general, there are four types of aflatoxinsB1, B2, G1 and G2, which among them aflatoxin B1 is the most potent carcinogen in animal species and causes liver cancer. The goal of this study was to evaluate of aflatoxin concentration in legumes samples marketed in Tehranbya high-performance liquid chromatography (HPLC). Fifty samples of legumes including peas, red beans, lentils, mung bean and cotyledons (n= 10 of pea,n= 10 of red bean,n= 10 of lentils,n= 10 of mung bean andn= 10 of cotyledons) were obtained from different regions (bulk legumes) of Tehran city in Iran and different packaging brands that had the highest supply in Tehran stores and were transported to the laboratory under appropriate conditions to measure aflatoxin (B1, B2, G1, G2 and total). Considering the limit of detection (LOD) of the current study, the aflatoxin level of all samples of peas, red beans, lentils, mung bean and cotyledons samples was lower than LOD. Based on the results, it can be concluded that the consumption of legumes in Tehran city by consumers can be safe, but more research is needed to avoid potential risks, and also routine and periodic monitoring of aflatoxin levels in legumes is recommended.

Origanum vulgare and Thymus vulgaris Extract Usability to Improve Silage Hygienic Quality and Reduce Mycotoxin Concentrations

JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY

Authors: Vaiciuliene, Gintare; Bakutis, Bronius; Jovaisiene, Jurgita; Falkauskas, Rimvydas; Gerulis, Gediminas; Baliukoniene, Violeta

Abstract

Silage is one of the main feed components of ruminants around the world and can make up about 50-80% of the rations of dairy cows during the winter. The aim of this study was to evaluate the usability of oregano (Origanum vulgare L.) and thyme (Thymus vulgaris L.) aqueous and ethanol extracts to improve the hygienic quality of perennial ryegrass, red clover and blue alfalfa silage samples and estimate their effect on mycotoxins concentrations. Under laboratory conditions, 63 silage samples (21 perennial ryegrass, 21 blue alfalfa, 21 red clover) were fermented with inserted aqueous and ethanol extracts of oregano and thyme and two commercial inoculants with mesophilic lactic acid bacteria. After 96 days of fermentation, in silage samples were established fermentation parameters, microbiological status and mycotoxins concentrations. It was determined that the best results for achieving hygienic quality of perennial ryegrass and red clover silage samples was by insertion of aqueous and ethanol extracts of oregano and thyme. In blue alfalfa samples, the best results of silage hygienic indicators were determined by inserting aqueous and ethanol extracts of oregano. Aflatoxin B1 (AFB1), deoxynivalenol (DON), zearalenone (ZEA) and T-2 toxin concentrations in perennial ryegrass, red clover and blue alfalfa silage samples were best reduced by inserting aqueous and ethanol extracts of oregano and thyme. The present study shows that these extracts can be used to improve silage hygienic quality, reduce mycotoxins concentrations and thus ensure the wellness of cattle.