Anti-Aflatoxin monoclonal antibody, clone BFA-3

The study aimed to clarify the characteristics of black tea (BTE) and/or curcumin (CMN) against aflatoxin-B1 (AFB1). Forty eight adult male Sprague-Dawley rats were divided into eight groups. G1 was non-treated control. G2, G3, and G4 were olive oil, BTE, and CMN, respectively. G5 was olive oil-dissolved AFB1 (25 mu g/kg b.w). G6, G7, and G8 were AFB1 along with BTE (2%), CMN (200 mg/kg b.w.), and BTE plus CMN, respectively. All treatments were orally given for consecutive 90 days. After treatment period, rats were sacrificed. Serobiochemical analysis and histopathology showed hepatorenal dysfunction in response to AFB1. Glutathione-antioxidants were significantly decreased versus increased lipid peroxides (p < .05-.001). AFB1 significantly increased the expression of the antitumor p53, but decreased that of antiapoptotic Bcl2 in liver or kidney tissue, either (p < .05). BTE or CMN ameliorated those changes induced by AFB1 in both liver and kidney with highly pronounced improvement when combined BTE/CMN was used. Practical applications Black tea (BTE) and curcumin (CMN) were known for their antioxidant effects, and several studies reported their independent effects against different toxicities including aflatoxicosis. The current study clarifies the ameliorative characteristics of both agents; BTE and/or CMN, against the toxicity resulted from the chronic exposure to aflatoxin-B1 (AFB1) (25 mu g/kg b.w. for consecutive 90 days). The dose of either agents, BTE or CMN, was 200 mg/kg b.w. along with AFB1. The pathologic changes, serobiochemical parameters, oxidative stress, histological changes, and the molecular disruption, induced by AFB1 in both liver and kidney were obviously and significantly ameliorated after BTE and/or CMN treatments in variable potencies where both agents showed the most effective antitoxic capacities.

Aflatoxin B-1 (AFB(1)) is one of mycotoxins leading to serious food contaminations and human diseases. To solve these problems, biodegrading technique has been concerned. Previous researches showed that AFB(1) can be effectively degraded by the culture supernatant of many microorganisms. However, the biodegraded products have not been identified. In this study, to explore the structure of the products, the AFB(1) was degraded by the culture supernatant of Cladosporium uredinicola. Two biodegraded products of AFB(1) were collected by high performance liquid chromatography (HPLC) and analyzed by liquid chromatography quadruple time-of-flight mass spectrometry (LC-Q-TOF MS) and chromatography-tandem mass spectrometry (MS/MS). As a result, the molecular weight of the two products were 406 and 342, and the structure of the two products were inferred. The toxicity of the biodegraded products was evaluated based on quantitative structure-activity relationship (QSAR) and cytotoxicity experiment. In conclusion, the double bonds of AFB(1) had a high probability of being destroyed. The toxicity of the two biodegraded products compared with that of AFB(1) was reduced. The culture supernatant of C. uredinicola was used as an effective way to detoxify AFB(1).