Aflatoxin M1 Residue ELISA Kit

Name: Aflatoxin M1 Residue ELISA Kit
SKU: DEIA2546
Rating: 5 (1 reviews)

Regulatory status: For research use only, not for use in diagnostic procedures.

COA

SDS

Datasheet

Summary

Sample

Dairy products

Detection Method

cELISA

Intended Use

This product can qualitatively and quantitatively detect the residual amount of aflatoxin M1 in raw milk, pasteurized milk, pure milk, modified milk, milk powder, yogurt, lactic acid bacteria beverage, chocolate-flavored milk sample, condensed milk, cheese, cream, solid cream and other samples.

Contents of Kit

No.	Components	Size	Storage Conditions
1	96-well ELISA plate (coated with conjugated antibody)	12×8	2-8°C
2	Aflatoxin M1 standard (0ppb, 0.005ppb, 0.01ppb, 0.025ppb, 0.05ppb, 0.1ppb, 0.2ppb)	1.5mL/bottle (1μg/L=1ppb=1μg/kg)	2-8°C
3	30× washing solution	15mL	2-8°C
4	Sample diluent	15mL	2-8°C
5	Aflatoxin M1 enzyme-labeled reagent	12mL	2-8°C
6	Substrate TMB	12mL	2-8°C
7	Stop solution	12mL	2-8°C

Storage

2-8°C

Performance Characteristics

Accuracy: 100±20%

Precision

CV value <10%

Detection Limit

1. Raw milk, pasteurized milk, pure milk, reconstituted milk, etc. 0.005 ppb
2. Milk powder 0.015 ppb
3. *Yogurt, lactic acid bacteria beverages, chocolate-flavored milk samples, condensed milk, etc. 0.025 ppb
4. Cheese, cream 0.05 ppb
5. Solid cream 0.1 ppb
*When yogurt, lactic acid bacteria beverages, chocolate-flavored milk samples, condensed milk, etc. are diluted 2 times, the detection limit can reach 0.01 ppb and the quantitative limit can reach 0.02 ppb.

Sensitivity

0.005 ppb

General Description

Aflatoxin M1 is a chemical compound of the aflatoxin class, a group of mycotoxins produced by three species of Aspergillus – Aspergillus flavus, Aspergillus parasiticus, and the rare Aspergillus nomius – which contaminate plant and plant products.
Aspergillus flavus produces only B-type aflatoxins. Aflatoxin M1 is the hydroxylated metabolite of aflatoxin B1 and can be found in milk or milk products obtained from livestock that have ingested contaminated feed. The carcinogenic potency of aflatoxin M1 in sensitive species is about one order of magnitude less than that of aflatoxin B1. Aflatoxin M1 is usually considered to be a detoxication by-product of aflatoxin B1. The main sources of aflatoxins in feeds are peanut, meal, maize and cottonseed meal.

Citations

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Documents

pdf COA

pdf SDS

pdf Datasheet

QA & Reviews

Customer Reviews

References

Origanum vulgare and Thymus vulgaris Extract Usability to Improve Silage Hygienic Quality and Reduce Mycotoxin Concentrations

JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY

Authors: Vaiciuliene, Gintare; Bakutis, Bronius; Jovaisiene, Jurgita; Falkauskas, Rimvydas; Gerulis, Gediminas; Baliukoniene, Violeta

Abstract

Silage is one of the main feed components of ruminants around the world and can make up about 50-80% of the rations of dairy cows during the winter. The aim of this study was to evaluate the usability of oregano (Origanum vulgare L.) and thyme (Thymus vulgaris L.) aqueous and ethanol extracts to improve the hygienic quality of perennial ryegrass, red clover and blue alfalfa silage samples and estimate their effect on mycotoxins concentrations. Under laboratory conditions, 63 silage samples (21 perennial ryegrass, 21 blue alfalfa, 21 red clover) were fermented with inserted aqueous and ethanol extracts of oregano and thyme and two commercial inoculants with mesophilic lactic acid bacteria. After 96 days of fermentation, in silage samples were established fermentation parameters, microbiological status and mycotoxins concentrations. It was determined that the best results for achieving hygienic quality of perennial ryegrass and red clover silage samples was by insertion of aqueous and ethanol extracts of oregano and thyme. In blue alfalfa samples, the best results of silage hygienic indicators were determined by inserting aqueous and ethanol extracts of oregano. Aflatoxin B1 (AFB1), deoxynivalenol (DON), zearalenone (ZEA) and T-2 toxin concentrations in perennial ryegrass, red clover and blue alfalfa silage samples were best reduced by inserting aqueous and ethanol extracts of oregano and thyme. The present study shows that these extracts can be used to improve silage hygienic quality, reduce mycotoxins concentrations and thus ensure the wellness of cattle.

Ochratoxin A and fumonisin B-1 exhibit synergistic cytotoxic effects by inducing apoptosis on rat liver cells

TOXICON

Authors: Wang, Haiyue; Wei, Yujia; Xie, Ying; Yan, Chao; Du, Hongzhen; Li, Zengning

Abstract

Aflatoxin G(1) (AFG(1)), ochratoxin A (OTA), fumonisin B-1 (FB1) and sterigmatocystin (ST) are very common toxic fungal metabolites with high detection rate and toxicity. The co-existence of many mycotoxins in animal feed has been reported worldwide, thus it is important to evaluate the combined cytotoxic effects among these mycotoxins. This study analysed the combined cytotoxic effects of the four mycotoxins in rat liver cells (BRL) and preliminary investigate their molecular mechanism. Cell viability assays were performed in accordance with the central composite design, and the combined index was analysed to estimate the combined effects. 4,6-Diamidino-2-phenylindole staining and flow cytometry analysis were performed, and Western blot analysis and quantitative real-time PCR were used to determine the molecular mechanism. The cytotoxicity of the mycotoxins on BRL was dose-dependent, following the order: ST > OTA > FB1 > AFG(1). OTA and FB1 presented synergetic cytotoxic effect on BRL by inducing apoptosis. Caspase-3 and Box expression were induced, whereas Bcl-2 was inhibited by treating with different concentrations of OTA and FB1. In addition, the Bax/Bcl-2 values were gradually increased, which indicated an increased degree of apoptosis. These results suggested that co-exposure of OTA and FB1 in agricultural products may be more hepatotoxic than OTA or FB1 separately.