Anti-AR (aa 221-320) polyclonal antibody

Several mechanisms regarding the developmental and reproductive toxicity of L-selenomethionine (SeMet) in freshwater fish have been widely reported. However, limited information was available on endocrine-disrupting effects of SeMet. In this study, zebrafish larvae (day 24 post-fertilization) were exposed to environmentally-relevant levels of dietary SeMet (control, 1.11 +/- 0.11, 3.59 +/- 0.20, 10.80 +/- 0.52, 29.19 +/- 0.46, and 58.63 +/- 0.70 mu g Se/g d.w. diet) for 90 days until sexual maturity. For the first time, a gender difference in the effect of SeMet on body mass of zebrafish was observed. The lowest observed adverse effect level (LOAEL) in male was 10.80 +/- 0.52 mu g Se/g d.w., while it was 29.19 +/- 0.46 mu g Se/g d.w. in female. Chronic exposure to dietary SeMet reduced the percentage of early vitellogenic oocyte in female and the percentage of spermatid in male by inhibiting the growth hormone/insulin-like growth factors (GH/IGFs) and hypothalamus-pituitary-gonad (HPG) systems, which was characterized by significant decreases in the transcriptional levels of gh, igf1, era, and ar. Se content in the liver of male was significantly higher than that in female when dietary Se level reached 10.80 +/- 0.52 mu g Se/g d.w.. Furthermore, the zinc content in the livers and gonads of both female and male treated with 29.19 +/- 0.46 and 58.63 +/- 0.70 mu g Se/g d.w. diets was significantly reduced. Reduction of zinc ion not only led to the significantly upregulated transcriptional levels of zip1 and znt2, but also downregulated transcriptional levels of znf219l and sp7. In summary, this study provides a new evidence that chronic exposure to high level of dietary SeMet (>= 10.80 mu g Se/g d.w.) could impair the development and reproduction of zebrafish with gender difference by interfering the GH/IGFs and HPG systems. Moreover, disruption of zinc ion homeostasis might exacerbate the toxicity of Se to zebrafish. (C) 2020 Elsevier Ltd. All rights reserved.

We report on searches for neutrinos and antineutrinos from astrophysical sources performed with the Borexino detector at the Laboratori Nazionali del Gran Sasso in Italy. Electron antineutrinos ((nu) over bar (e)) are detected in an organic liquid scintillator through the inverse beta-decay reaction. In the present work we set model-independent upper limits in the energy range 1.8-16.8 MeV on neutrino fluxes from unknown sources that improve our previous results, on average, by a factor 2.5. Using the same data set, we first obtain experimental constraints on the diffuse supernova (nu) over bar (e) fluxes in the previously unexplored region below 8 MeV. A search for (nu) over bar (e) in the solar neutrino flux is also presented: the presence of (nu) over bar (e) would be a manifestation of a non-zero anomalous magnetic moment of the neutrino, making possible its conversion to antineutrinos in the strong magnetic field of the Sun. We obtain a limit for a solar (nu) over bar (e) flux of 384 cm(-2) s(-1) (90% C.L.), assuming an undistorted solar 8B neutrinos energy spectrum, that corresponds to a transition probability p(nu e ->(nu) over bare)< 7.2 x 10-5 (90% C.L.) for E-<(nu)over bar>e > 1.8 MeV. At lower energies, by investigating the spectral shape of elastic scattering events, we obtain a new limit on solar Be-7-nu(e) conversion into (nu) over bar (e) of p(nu e ->(nu) over bare)< 0.14 (90% C.L.) at 0.862 MeV. Last, we investigate solar flares as possible neutrino sources and obtain the strongest up-to-date limits on the fluence of neutrinos of all flavor neutrino below 3-7 MeV. Assuming the neutrino flux to be proportional to the flare's intensity, we exclude an intense solar flare as the cause of the observed excess of events in run 117 of the Cl-Ar Homestake experiment. (C) 2020 Elsevier B.V. All rights reserved.