Androgen Receptor (Phospho-Ser650) ELISA Kit

Anticoagulant rodenticides (ARs) are commonly used to control rodent pests. However, worldwide, their use is associated with secondary and tertiary poisoning of nontarget species, especially predatory and scavenging birds. No medical device can rapidly test for AR exposure of avian wildlife. Prothrombin time (PT) is a useful biomarker for AR exposure, and multiple commercially available point-of-care (POC) devices measure PT of humans, and domestic and companion mammals. We evaluated the potential of one commercially available POC device, the Coag-Sense PT/INR Monitoring System, to rapidly detect AR exposure of living birds of prey. The Coag-Sense device delivered repeatable PT measurements on avian blood samples collected from four species of raptors trapped during migration (Intraclass Correlation Coefficient > 0.9; overall intra-sample variation CV: 5.7%). However, PT measurements reported by the Coag-Sense system from 81 ferruginous hawk (Buteo regalis) nestlings were not correlated to those measured by a one-stage laboratory avian PT assay (r = - 0.017, p = 0.88). Although precise, the lack of agreement in PT estimates from the Coag-Sense device and the laboratory assay indicates that this device is not suitable for detecting potential AR exposure of birds of prey. The lack of suitability may be related to the use of a mammalian reagent in the clotting reaction, suggesting that the device may perform better in testing mammalian wildlife

In this study, the solution preparation and the annealing conditions were studied for spin-coated ITO films. Then, the effect of several post-treatment parameters such as H-2 concentration, temperature, and processing time were investigated for the H-2/Ar atmosphere. The properties of thin films were characterized by four-probe measurement set-up, UV-visible spectroscopy (UV-Vis), scanning electron microscopy (SEM), X-ray diffractometer (XRD) and X-ray photoelectron spectroscopy (XPS). It has been found that seven times deposition of 0.5 M solution including In:Sn with the ratio of 90:10 and annealing the films at 600 degrees C in air for 30 min was the optimum preparation condition for ITO films. Subjecting the ITO films to 10% H-2 containing Ar medium at 300 degrees C for 3 h was found as the most efficient process for post-treatment and as a result of that, approximately 70% efficiency was achieved on the reduction of the sheet resistance without affecting the transmittance of the thin films. (C) 2020 Elsevier B.V. All rights reserved.