Androgen Receptor (Phospho-Ser94) ELISA Kit

Name: Androgen Receptor (Phospho-Ser94) ELISA Kit
SKU: DEIA-XYA858
Rating: 5 (1 reviews)

Regulatory status: For research use only, not for use in diagnostic procedures.

COA

SDS

Datasheet

Summary

Sample

cultured cells

Species Reactivity

Human; Mouse

Detection Method

Indirect ELISA

Intended Use

The Androgen Receptor (Phospho-Ser94) Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor Androgen Receptor protein phosphorylation and expression profile in cells. The kit can be used for measuring the relative amounts of phosphorylated Androgen Receptor in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on Androgen Receptor phosphorylation.

Contents of Kit

1. 96-Well Cell Culture Clear-Bottom Microplate: 2 plates
2. 10x TBS: 24 mL
3. Quenching Buffer: 24 mL
4. Blocking Buffer: 50 mL
5. 10x Wash Buffer: 50 mL
6. 100x Anti-Androgen Receptor (Phospho-Ser94) Antibody (Rabbit Polyclonal): 60 μL, red
7. 100x Anti-Androgen Receptor Antibody (Rabbit Polyclonal): 60 μL, purple
8. 100x Anti-GAPDH Antibody (Mouse Monoclonal): 60 μL, green
9. HRP-Conjugated Anti-Rabbit IgG Antibody: 12 mL, glass
10. HRP-Conjugated Anti-Mouse IgG Antibody: 12 mL, glass
11. Primary Antibody Diluent: 12 mL
12. Ready-to-Use Substrate: 12 mL
13. Stop Solution: 12 mL
14. Crystal Violet Solution: 12 mL
15. SDS Solution: 24 mL
16. Adhesive Plate Seals: 4 seals

Storage

4°C/6 Months

Citations

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Documents

pdf COA

pdf SDS

pdf Datasheet

QA & Reviews

Customer Reviews

References

Demonstration of Transmission Mode Soft X-ray NEXAFS Using Third- and Fifth-Order Harmonics of FEL Radiation at SACLA BL1

APPLIED SCIENCES-BASEL

Authors: Iwayama, Hiroshi; Nagasaka, Masanari; Inoue, Ichiro; Owada, Shigeki; Yabashi, Makina; Harries, James R.

Abstract

We demonstrate the applicability of third- and fifth-order harmonics of free-electron laser (FEL) radiation for soft X-ray absorption spectroscopy in the transmission mode at SACLA BL1, which covers a photon energy range of 20 to 150 eV in the fundamental FEL radiation. By using the third- and fifth-order harmonics of the FEL radiation, we successfully recorded near-edge X-ray absorption fine structure (NEXAFS) spectra for Ar 2p core ionization and CO2 C 1s and O 1s core ionizations. Our results show that the utilization of third- and fifth-order harmonics can significantly extend the available photon energies for NEXAFS spectroscopy using an FEL and opens the door to femtosecond pump-probe NEXAFS using a soft X-ray FEL.

Study on dehydrogenation behaviour of molten steel in single snorkel refining furnace (SSRF) by a mathematical model

IRONMAKING & STEELMAKING

Authors: Zhang, Guo-lei; Cheng, Guo-guang; Dai, Wei-xing; Huang, Yu; Wang, Qi-ming

Abstract

A mathematical model of an industrial Single Snorkel Refining Furnace (SSRF) has been developed to investigate dehydrogenation behaviour in the vacuum refining process. Dehydrogenation reactions were considered to take place at three sites: Ar bubble surface, bath surface, and the bulk steel. The effects of Ar bubble behaviour and H2 heterogeneous nucleation behaviour of the bulk steel on dehydrogenation were taken into account specifically. The simulation results are in good agreement with the measured industrial data. Using the model, the dehydrogenation rate at different sites and its contribution to total dehydrogenation value were evaluated in detail. The results indicated that bulk steel dehydrogenation occurred only the high concentration range, while the other two reactions participated in the whole refining process. In addition, the effect of argon flow rate on the dehydrogenation rate was also obtained and the reasonable range of argon flow rate for SSRF treatment has been recommended.