SARS-CoV-2 Spike RBD Protein (G476S) [His]

Product Overview

A DNA sequence encoding the SARS-CoV-2 Spike RBD(G476S)-His Recombinant Protein (YP_009724390.1) (Arg319-Phe541(G476S)) was expressed with a polyhistidine tag at the C-terminus.

Nature

Recombinant

Predicted N terminal

Arg319

Tag/Conjugate

His

Molecular Weight

The recombinant SARS-CoV-2 Spike RBD(G476S)-His Recombinant Protein consists of 234 amino acids and predicts a molecular mass of 26.57 kDa.

Endotoxin

< 1.0 EU per ug protein as determined by the LAL method.

Alternative Names

SARS-CoV-2; SARS-CoV-2 Spike RBD Protein; SARS-CoV-2 RBD; SARS-CoV-2 S1 RBD; SARS-CoV-2 RBD Protein

Purity

> 95 % as determined by SDS-PAGE.

Format

Lyophilized

Buffer

Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.

Preservative

None

Storage

Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Ship

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Introduction

The spike (S) glycoprotein of coronaviruses contains protrusions that will only bind to certain receptors on the host cell: they are essential for both host specificity and viral infectivity. The term 'peplomer' is typically used to refer to a grouping of heterologous proteins on the virus surface that function together. The spike (S) glycoprotein of coronaviruses is known to be essential in the binding of the virus to the host cell at the advent of the infection process. Most notable is severe acute respiratory syndrome (SARS). The severe acute respiratory syndrome-coronavirus (SARS-CoV) spike (S) glycoprotein alone can mediate the membrane fusion required for virus entry and cell fusion. It is also a major immunogen and a target for entry inhibitors. The SARS-CoV spike (S) protein is composed of two subunits; the S1 subunit contains a receptor-binding domain that engages with the host cell receptor angiotensin-converting enzyme 2 and the S2 subunit mediates fusion between the viral and host cell membranes. The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity, during infection with SARS-CoV.

Keywords

SARS-CoV-2; SARS-CoV-2 Spike RBD Protein; SARS-CoV-2 RBD; SARS-CoV-2 S1 RBD; SARS-CoV-2 RBD Protein