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SARS-CoV-2 Mutants Protein

Genetic variants of SARS-CoV-2 have been emerging and circulating around the world throughout the COVID-19 pandemic. The U.K. variant B.1.1.7, the Brazil variant P.1, the South Africa variant B.1.351, and the most recent Indian variant B.1.617 are particular concerning because of their high prevalence.

While the Spike protein in each variant has several mutations, there are three that are of particular concern. The N501Y mutation occurs in the receptor binding domain of the spike protein, positioned to influence binding to the host ACE2 receptor. The E484K mutation is found in both the South African strain (B.1.1.7) and the one that is now prevalent in Brazil (P.1). There is growing evidence that this mutation may diminish neutralizing effects of some antibodies. K417 has mutated to N in the B.1.351 lineage, while it changed to T (K417T) in the P1 lineage identified in Brazil.

The mutations in these strains also occur in the nucleocapsid protein, which is commonly used as the biomarker in rapid antigen tests. It’s critical to assess whether the current commercial antigen tests can detect the mutated N proteins with the same sensitivity and specificity as their WT counterpart.

Creative Diagnostics are going full steam ahead on developing a collection of recombinant antigens for these variants, including Spike proteins and Nucleocapsid proteins, covering critical mutations such as K417N/T, E484K, N501Y and D614G on Spike protein and R203G, G204R and P13L on Nucleocapsid protein. The reagents can be used to evaluate the efficacy of the antibodies and vaccination.

Recombinant Mutants from the India Variant | B.1.617
Cat. No Mutations Antigen Tag
DAGC583 L452R, E484Q RBD His
DAGC665 L452R, T478K RBD hFc
DAGC376 L452R RBD His
DAGC585 E484Q RBD His
DAGC594 D614G, E154K, E484Q, L452R, P681R S1 His
DAGC669 T95I, G142D, E154K, L452R, E484Q, D614G, P681R S1 His
DAGC670 T19R, G142D, E156G, del157/158, L452R, T478K, D614G, P681R S1 His
DAGC671 T19R, L452R, E484Q, D614G, P681R, G142D S1 His
DAGC595 D614G, E154K, E484Q, E1072K, K1073R, L452R, P681R S1+S2 ECD His
DAGC596(under development) D614G, E154K, E484Q, G142D, H1101D, L452R, P681R, Q1071H S1+S2 ECD His
DAGC666(under development) T95I, G142D, E154K, L452R, E484Q, D614G, P681R, Q1071H S1+S2 ECD His
DAGC667(under development) T19R, G142D, del157/158, L452R, T478K, D614G, P681R, D950N S1+S2 ECD His
DAGC668(under development) T19R, L452R, E484Q, D614G, P681R, D950N, G142D S1+S2 ECD His
DAGC597 R203M, D377Y N His
DAGC598 D377Y N His
DAGC674 D63G, R203M, D377Y N His
DAGC675 P67S, R203M, D377Y N His
Recombinant Mutants from the U.K. Variant | B.1.1.7
Cat. No Mutations Antigen Tag
DAGC476 N501Y RBD His
DAGC501 N501Y RBD His, Biotinylated
DAGC602 N501Y RBD rFc
DAGC503 HV69-70 deletion, N501Y, D614G S1 His
DAGC488 HV69-70/Y144 deletion, N501Y, A570D, D614G, P681H S1 His
DAGC502 HV69-70/Y144 deletion, N501Y, A570D, D614G, P681H S1 His, Biotinylated
DAGC506 HV69-70/Y144 deletion, N501Y, A570D, D614G, P681H, T716I, S982A, D1118H S1+S2 ECD His
DAGC504 D3L, R203K, G204R, S235F N His
DAGC505 D3L, S235F N His
DAGC676 E378Q N His
DAGC515 R203K, G204R N His
DAGC516 P13L N His
DAGC517 S194L N His
DAGC518 I292T N His
Recombinant Mutants from the South Africa Variant | B.1.351
Cat. No Mutations Antigen Tag
DAGC476 N501Y RBD His
DAGC501 N501Y RBD His, Biotinylated
DAGC507 E484K RBD His
DAGC508 E484K RBD His, Biotinylated
DAGC404 K417N RBD His
DAGC510 K417N RBD His, Biotinylated
DAGC511 K417N, E484K, N501Y RBD His
DAGC512 K417N, E484K, N501Y RBD His, Biotinylated
DAGC603 E484K RBD rFc
DAGC604 K417N, E484K, N501Y RBD rFc
DAGC677 K417N, E484K, N501Y RBD hFc
DAGC605 K417N RBD rFc
DAGC489 K417N, E484K, N501Y, D614G S1 His
DAGC682 K417N, E484K, N501Y, D614G S1 His, Biotinylated
DAGC683 L18F, D80A, D215G, LAL242-244 deletion, R246I, K417N, E484K, N501Y, D614G S1 His
DAGC684 L18F, D80A, D215G, LAL242-244 deletion, R246I S1 NTD His
DAGC678 D80A, LAL242-244 deletion, R246I, E484K, K417N, N501Y, D614G, A701V S1+S2 ECD His
DAGC679 D80A, K417N, E484K, N501Y, D614G, A701V S1+S2 ECD His
DAGC680 L18F, D80A, D215G, LAL242-244 deletion, R246I, K417N, E484K, N501Y, D614G, A701V S1+S2 ECD His
DAGC681 L18F, D80A, D215G, LAL242-244 deletion, R246I, K417N, E484K, N501Y, D614G, A701V S1+S2 ECD His, Biotinylated
DAGC650 T205I N His
DAGC685(under development) T205I, A220V, P13L N His
Recombinant Mutants from the Brazil Variant | P.1
Cat. No Mutations Antigen Tag
DAGC476 N501Y RBD His
DAGC501 N501Y RBD His, Biotinylated
DAGC507 E484K RBD His
DAGC508 E484K RBD His, Biotinylated
DAGC587 K417T, E484K, N501Y RBD His
DAGC588 K417T, E484K, N501Y RBD rFc
DAGC686 K417T, E484K, N501Y RBD hFc
DAGC590 K417T RBD rFc
DAGC687 L18F, T20N, P26S, D138Y, R190S, K417T, E484K, N501Y, D614G, H655Y S1 His
DAGC688 T20N, D614G S1 His
DAGC589 L18F, T20N, P26S, D138Y, R190S, K417T, E484K, N501Y, D614G, H655Y, T1027I, V1176F S1+S2 ECD His
DAGC689 L18F, T20N, P26S, D138Y, R190S, K417T, E484K, N501Y, D614G, H655Y, T1027I S1+S2 ECD His
DAGC690 P80R N His
Recombinant Mutants from the Denmark Mink Variant
Cat. No Mutations Antigen Tag
DAGC413 Y453F RBD His
DAGC514 HV69-70 deletion, Y453F, D614G S1 His
DAGC606 Y453F RBD rFc
Recombinant Mutants from the California Variant | B.1.429
Cat. No Mutations Antigen Tag
DAGC649 W152C, L452R, D614G S1 His
DAGC650 T205I N His
Other S& N Mutants
Cat. No Mutations Antigen Tag
DAGC186 V367F RBD His
DAGC187 K458R RBD His
DAGC188 F342L RBD His
DAGC189 V483A RBD His
DAGC190 A435S RBD His
DAGC191 N354D RBD His
DAGC217 N354D, D364Y RBD His
DAGC218 R408I RBD His
DAGC219 W436R RBD His
DAGC238 V367F RBD mFc
DAGC239 G476S RBD His
DAGC361 V341I RBD His
DAGC362 Y508H RBD His
DAGC363 N439K RBD His
DAGC364 V503F RBD His
DAGC365 A522V RBD His
DAGC366 S494P RBD His
DAGC367 A372S RBD His
DAGC368 A520S RBD His
DAGC369 A522S RBD His
DAGC370 D405V, Q414A RBD His
DAGC371 Q414E RBD His
DAGC372 P384L RBD His
DAGC373 A348S RBD His
DAGC374 F338L RBD His
DAGC375 F377L RBD His
DAGC377 P521S RBD His
DAGC378 T478I RBD His
DAGC379 V483I RBD His
DAGC380 S359N RBD His
DAGC381 K378R RBD His
DAGC382 Q409E RBD His
DAGC383 I472V RBD His
DAGC384 A372T RBD His
DAGC385 A344S RBD His
DAGC386 A520V RBD His
DAGC387 E406Q RBD His
DAGC388 F490S RBD His
DAGC389 K378N RBD His
DAGC390 N370S RBD His
DAGC391 Q414R RBD His
DAGC392 S477I RBD His
DAGC393 S477N RBD His
DAGC394 T385A RBD His
DAGC395 T393P RBD His
DAGC396 V395I RBD His
DAGC397 G485S RBD His
DAGC398 G482S RBD His
DAGC399 K444R RBD His
DAGC400 N440K RBD His
DAGC401 E471Q RBD His
DAGC402 P479S RBD His
DAGC403 A352S RBD His
DAGC405 P337S RBD His
DAGC406 P521R RBD His
DAGC407 S477R RBD His
DAGC479 D614G, E484K RBD sFc
DAGC210 D614G S1 His
DAGC359 D614G S1 hFc
DAGC594 D614G, E154K, E484Q, L452R, P681R S1 His
DAGC358 S577A S1+S2 ECD His
DAGC360 R683A, R685A, F817P, A892P, A899P, A942P, K986P, V987P S1+S2 ECD His
DAGC595 D614G, E154K, E484Q, E1072K, K1073R, L452R, P681R S1+S2 ECD His
DAGC596 D614G, E154K, E484Q, G142D, H1101D, L452R, P681R, Q1071H S1+S2 ECD His
DAGC597 R203M, D377Y N His
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