SARS-CoV-2 S protein (R683A, R685A) [His] (DAGC213)

Recombinant SARS-CoV-2 S protein (R683A, R685A) from HEK293 Cells [His]

Product Overview
SARS-CoV-2 S protein (R683A, R685A), His Tag is expressed from human 293 cells (HEK293). It contains AA Val 16 - Pro 1213 (Accession # QHD43416.1 (R683A, R685A)). This protein carries a polyhistidine tag at the C-terminus.
Nature
Recombinant
Predicted N terminal
Val 16
Tag/Conjugate
His
Molecular Weight
The protein has a calculated MW of 134.6 kDa. The protein migrates as 170-220 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 1.0 EU per ug by the LAL method.
Alternative Names
SARS-CoV-2; SARS-CoV-2 Spike Protein; SARS-CoV-2 S Protein
Procedure
None
Purity
>85% as determined by SDS-PAGE.
Format
Lyophilized
Size
100ug, 1mg
Buffer
Lyophilized from 0.22 um filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.
Preservative
None
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
Introduction
It's been reported that SARS-CoV-2 can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
Keywords
SARS-CoV-2; SARS-CoV-2 Spike Protein; SARS-CoV-2 S Protein

Data Examples


SARS-CoV-2 S protein (R683A, R685A), His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 85%.

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